Sousa Renata P, Duarte Ana Beatriz G, Pinto Yago, Sá Naiza A R, Alves Benner G, Cibin Francielli W S, Silva Gustavo C, Carvalho Camila E S, Argôlo Neto Napoleão M, Rodrigues Ana Paula R, Silva Cleidson M G, Figueiredo José R, Carvalho Maria Acelina M
Integrated Nucleus of Morphology and Research with Stem Cells (NUPCelt), Federal University of Piauí, Teresina, PI, Brazil.
Department of Morphology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Reprod Sci. 2021 Jun;28(6):1709-1717. doi: 10.1007/s43032-021-00540-3. Epub 2021 Mar 15.
The development of culture systems capable of maintaining follicular growth since the preantral stage has been the target of investigations. Mesenchymal stem cells (MSC) present potential for use in a wide range of applications, including research aimed at preserving fertility. Therefore, this study investigated the use of caprine Wharton's Jelly Mesenchymal Stem Cells (WJMSC) on the survival and in vitro development of goat preantral follicles enclosed in ovarian fragments cultured for 1 or 7 days. Fragments of the ovarian cortex were immediately fixed (non-cultured control) or distributed in four treatments: ovarian tissue cultured in control medium (α-MEM); ovarian tissue cultured in α-MEM supplemented with FBS (α-MEM + FBS); ovarian tissue co-cultured with stem cells in α-MEM (α-MEM + SC); and ovarian tissue co-cultured with stem cell in α-MEM + FBS (α-MEM + SC + FBS). The rates of cell proliferation, follicular survival, and activation, as well as follicular diameter, were evaluated. After 7 days, the treatment co-cultured with stem cells showed a higher (P < 0.05) percentage of morphologically normal preantral follicles compared to the other treatments, as well as a higher (P < 0.05) activation rate compared to cultured control. Moreover, the follicular diameter was higher (P < 0.05) in the treatment co-cultured with stem cells compared to co-cultured with stem cells plus FBS. This study demonstrates for the first time that in vitro co-culture of caprine WJMSC with preantral follicles enclosed in goat ovarian tissue improves activation and early follicular development.
自窦前阶段起就能够维持卵泡生长的培养系统的开发一直是研究的目标。间充质干细胞（MSC）在广泛的应用中具有使用潜力，包括旨在保护生育能力的研究。因此，本研究调查了山羊脐带华通氏胶间充质干细胞（WJMSC）对培养1天或7天的卵巢碎片中所包含的山羊窦前卵泡的存活及体外发育的作用。卵巢皮质碎片立即固定（非培养对照）或分为四种处理：在对照培养基（α-MEM）中培养的卵巢组织；在添加胎牛血清的α-MEM（α-MEM + FBS）中培养的卵巢组织；在α-MEM中与干细胞共培养的卵巢组织（α-MEM + SC）；以及在α-MEM + FBS中与干细胞共培养的卵巢组织（α-MEM + SC + FBS）。评估了细胞增殖率、卵泡存活率和激活率以及卵泡直径。7天后，与其他处理相比，与干细胞共培养的处理显示形态学正常的窦前卵泡百分比更高（P < 0.05），与培养对照相比激活率也更高（P < 0.05）。此外，与干细胞加FBS共培养相比，与干细胞共培养的处理中卵泡直径更高（P < 0.05）。本研究首次证明，山羊WJMSC与山羊卵巢组织中所包含的窦前卵泡进行体外共培养可改善激活和早期卵泡发育。
