A new technique for subchoroidal implantation of experimental malignant melanoma.

A new technique for implanting Greene hamster amelanotic melanoma cells into the rabbit eye is described. The technique involves the deposition of a tumor fragment into the subchoroidal space via a transvitreal approach. Thirty rabbit eyes were implanted with 26 successful tumor growths producing solitary choroidal nodules. This technique offers the advantages of rapid implantation, the ability to precisely choose the site of implantation including posterior sites, and eliminates the need for a large scleral incision.