Faculty of Veterinary Medicine, Department of Internal Medicine, Hatay Mustafa Kemal University, Hatay, Turkey.
Sifa Bioresonance & Clinical Physiology Center, Kocaeli, Turkey.
Biotech Histochem. 2022 Feb;97(2):90-98. doi: 10.1080/10520295.2021.1896780. Epub 2021 Mar 16.
Gastric outlet obstruction (GOO) is caused mainly by pyloric or duodenal blockage; gastric surgery and vagotomy are effective treatments. We investigated the short term effects of experimental GOO and truncal vagotomy (TV) on gut hormone levels. We used 8-week-old male Wistar rats divided randomly into four groups: control, GOO, TV, and GOO + TV. At the end of the experiment, blood and tissue samples of the pylorus and fundus were obtained for biochemical and immunohistochemical analysis. Gastric motility decreased in the TV group, but there was no difference in food intake compared to the control group; water consumption and urine output were increased. Feces excretion and food intake decreased due to loss of food movement from the stomach of GOO and GOO + TV rats. Levels of insulin and ghrelin were lower than for the control group, but levels of cholecystokinin were higher. Leptin and glucagon-like peptide 1 levels were increased in the GOO group, while somatostatin was decreased. Leptin immunostaining levels were decreased in the GOO + TV group. Gastrin and neuropeptide Y levels were lower in the GOO and GOO + TV groups compared to the other groups. We found that both gut hormone levels related to gastric motility and metabolism, and immunohistochemical staining of the stomach tissue were altered by TV and GOO. Measuring changes in gut hormones following gastric surgery could be useful for monitoring the effectiveness of treatment.
胃出口梗阻（GOO）主要由幽门或十二指肠阻塞引起；胃手术和迷走神经切断术是有效的治疗方法。我们研究了实验性 GOO 和全胃迷走神经切断术（TV）对肠道激素水平的短期影响。我们使用 8 周龄雄性 Wistar 大鼠随机分为四组：对照组、GOO 组、TV 组和 GOO + TV 组。在实验结束时，采集幽门和胃底的血液和组织样本进行生化和免疫组织化学分析。TV 组的胃动力下降，但与对照组相比，食物摄入量没有差异；水和尿的排出量增加。GOO 和 GOO + TV 大鼠由于胃内食物运动丧失，粪便排泄和食物摄入量减少。胰岛素和胃饥饿素水平低于对照组，但胆囊收缩素水平升高。GOO 组的瘦素和胰高血糖素样肽 1 水平升高，而生长抑素水平降低。GOO + TV 组的瘦素免疫染色水平降低。与其他组相比，GOO 和 GOO + TV 组的胃泌素和神经肽 Y 水平较低。我们发现，与胃动力和代谢相关的肠道激素水平以及胃组织的免疫组织化学染色均因 TV 和 GOO 而改变。测量胃手术后肠道激素的变化可能有助于监测治疗效果。
