环状 RNA 作为竞争内源性 RNA 在急性髓系白血病发病机制中的特征。

The characteristics of circRNA as competing endogenous RNA in pathogenesis of acute myeloid leukemia.

机构信息

School of Medicine, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, 710061, Shaanxi, China.

出版信息

BMC Cancer. 2021 Mar 15;21(1):277. doi: 10.1186/s12885-021-08029-7.

BACKGROUND

As one of the novel molecules, circRNA has been identified closely involved in the pathogenesis of many diseases. However, the function of circRNA in acute myeloid leukemia (AML) still remains unknown.

METHODS

In the current study, the RNA expression profiles were obtained from Gene Expression Omnibus (GEO) datasets. The differentially expressed RNAs were identified using R software and the competing endogenous RNA (ceRNA) network was constructed using Cytoscape. Functional and pathway enrichment analyses were performed to identify the candidate circRNA-mediated aberrant signaling pathways. The hub genes were identified by MCODE and CytoHubba plugins of Cytoscape, and then a subnetwork regulatory module was established.

RESULTS

A total of 27 circRNA-miRNA pairs and 208 miRNA-mRNA pairs, including 12 circRNAs, 24 miRNAs and 112 mRNAs were included in the ceRNA network. Subsequently, a subnetwork, including 4 circRNAs, 5 miRNAs and 6 mRNAs, was established based on related circRNA-miRNA-mRNA regulatory modules.

CONCLUSIONS

In summary, this work analyzes the characteristics of circRNA as competing endogenous RNA in AML pathogenesis, which would provide hints for developing novel prognostic, diagnostic and therapeutic strategy for AML.

背景

circRNA 作为新型分子之一，其在许多疾病的发病机制中密切相关已得到确认。然而，circRNA 在急性髓系白血病（AML）中的功能仍然未知。

方法

在本研究中，从基因表达综合数据库（GEO）数据集获得 RNA 表达谱。使用 R 软件鉴定差异表达的 RNA，并使用 Cytoscape 构建竞争内源性 RNA（ceRNA）网络。进行功能和通路富集分析以鉴定候选 circRNA 介导的异常信号通路。使用 Cytoscape 的 MCODE 和 CytoHubba 插件鉴定枢纽基因，然后建立子网调控模块。

结果

ceRNA 网络包括 27 个 circRNA-miRNA 对和 208 个 miRNA-mRNA 对，包括 12 个 circRNAs、24 个 miRNAs 和 112 个 mRNAs。随后，基于相关 circRNA-miRNA-mRNA 调控模块，建立了一个包括 4 个 circRNAs、5 个 miRNAs 和 6 个 mRNAs 的子网。