Department of Biotechnology and Cell Biology, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland.
Department of Biotechnology and Cell Biology, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland.
Toxicol In Vitro. 2021 Jun;73:105143. doi: 10.1016/j.tiv.2021.105143. Epub 2021 Mar 12.
Triclosan (TCS) is a well-known compound that can be found in disinfectants, personal care products. There is one publication concerning the involvement of PPARγ in the mechanism of action of TCS. It is known that activation of PPARγ regulates the expression of the NF-κB mediated inflammation by acting on nitric oxide synthase (NOS) genes. However, there are no studies demonstrating a relationship between the effects of TCS on the PPARγ signaling pathway, changes in NF-κB expression, and NOS isoform synthesis. Therefore, the aim of this study was to evaluate the effect of TCS on the expression of PPARγ, NF-κB, nNOS, iNOS, and eNOS in mouse neocortical neurons. In addition, the effects of co-administration of synthetic alpha-naphthoflavone (αNF) or beta-naphthoflavone (βNF) flavonoids and triclosan were investigated. Our results show that TCS alters PPARγ, NF-κB, iNOS, and eNOS expression in mouse neurons in vitro. After 48 h of exposure, TCS increased PPARγ expression and decreased NF-κB expression. Moreover, under the influence of TCS, the expression of iNOS was increased and at the same time the expression of nNOS was decreased, which was probably caused by high levels of ROS. The experiments have shown that both αNF and βNF are able to modulate the effects of TCS in primary cultures of mouse cortical neurons.
三氯生（TCS）是一种众所周知的化合物，可在消毒剂、个人护理产品中找到。有一篇关于 PPARγ参与 TCS 作用机制的出版物。已知 PPARγ 的激活通过作用于一氧化氮合酶（NOS）基因来调节 NF-κB 介导的炎症的表达。然而，没有研究表明 TCS 对 PPARγ 信号通路的影响、NF-κB 表达的变化和 NOS 同工型合成之间存在关系。因此，本研究旨在评估 TCS 对小鼠新皮质神经元中 PPARγ、NF-κB、nNOS、iNOS 和 eNOS 表达的影响。此外，还研究了合成α-萘黄酮（αNF）或β-萘黄酮（βNF）类黄酮与三氯生共同给药的影响。我们的结果表明，TCS 改变了体外培养的小鼠神经元中 PPARγ、NF-κB、iNOS 和 eNOS 的表达。暴露 48 小时后，TCS 增加了 PPARγ 的表达并降低了 NF-κB 的表达。此外，在 TCS 的影响下，iNOS 的表达增加，同时 nNOS 的表达减少，这可能是由于高水平的 ROS 引起的。实验表明，αNF 和 βNF 均能够调节 TCS 在小鼠皮质神经元原代培养物中的作用。
