Department of Zoology, School of Life Sciences, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur, Chhattisgarh, India.
Department of Zoology, School of Life Sciences, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur, Chhattisgarh, India.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Jul;245:109029. doi: 10.1016/j.cbpc.2021.109029. Epub 2021 Mar 17.
The utilization of pesticides has increased for destroying pests and protecting crops in the agriculture field. Triazophos is a commonly used organophosphorous insecticide that causes alterations in haematological and histological parameters in fish. The present study was designed to evaluate the effect of triazophos induced innate and cell mediated immunotoxicity in freshwater teleost, Channa punctata. Fishes were exposed to triazophos at concentrations 5 and 10% of LC value for 10 and 20 days. Splenic and head kidney macrophage phagocytosis, nitric oxide production and superoxide production were assayed to evaluate the innate immunity. Cell-mediated immunity was measured through splenic and head kidney lymphocyte proliferation in presence of T and B cell mitogens. Results of the present study revealed that macrophage phagocytosis was significantly reduced after in vivo triazophos treatment. Differential suppressive effect of triazophos was also observed where mitogen induced splenic and head kidney lymphocyte proliferations were reduced after 10 and 20 days treatment. Concentration dependent effect of triazophos was observed in in vivo studies where the production of reactive oxygen and nitrogen intermediates were suppressed. This study describes the first investigation of the effect of triazophos on immune functions and will help to determine appropriate ecotoxicity and immunotoxicity in freshwater teleosts.
在农业领域，为了消灭害虫和保护作物，人们越来越多地使用农药。三唑磷是一种常用的有机磷杀虫剂，它会导致鱼类血液学和组织学参数发生变化。本研究旨在评估三唑磷对淡水硬骨鱼 Channa punctata 固有和细胞介导免疫毒性的影响。将鱼类暴露于 LC 值的 5%和 10%的三唑磷浓度下 10 和 20 天。测定了脾脏和头肾巨噬细胞的吞噬作用、一氧化氮产生和超氧化物产生，以评估固有免疫。通过在 T 和 B 细胞有丝分裂原存在下测定脾脏和头肾淋巴细胞的增殖来测量细胞介导的免疫。本研究的结果表明，体内三唑磷处理后巨噬细胞的吞噬作用显著降低。三唑磷还表现出差异抑制作用，在 10 和 20 天处理后，有丝分裂原诱导的脾脏和头肾淋巴细胞增殖减少。体内研究观察到三唑磷的浓度依赖性效应，其中活性氧和氮中间产物的产生受到抑制。本研究首次描述了三唑磷对免疫功能的影响，将有助于确定淡水硬骨鱼的适当生态毒性和免疫毒性。
