Department of Pathology, Diamond Harbour Government Medical College, Diamond Harbour, West Bengal, India.
Department of Pathology, Mission of Mercy Hospital, Durgapur, West Bengal, India.
J Cancer Res Ther. 2021 Jan-Mar;17(1):164-169. doi: 10.4103/jcrt.JCRT_311_19.
Ovarian cancer is the fifth common cause of death due to cancer in women. It constitutes 3% of all cancers in females and 15%-20% of genital malignancy. Most of the ovarian cancers are serous type followed by the endometrioid type. Sometimes, glands of these two carcinomas are indistinguishable histologically. It also becomes difficult to differentiate these two types when they are poorly differentiated.
The aim of this study was to find differences in immunomarker expressions between serous and endometrioid carcinomas and the association of their staining patterns with other clinicopathological prognostic factors.
Immunohistochemical staining for WT1, B-cell lymphoma 2 (Bcl2), Ki67 (MIB1), and Her2/Neu were done in paraffin-embedded tissues of histologically diagnosed 38 cases of ovarian serous and endometrioid carcinomas and staining patterns were correlated with other clinicopathological prognostic factors.
Of these 38 cases (21 serous and 17 endometrioid), 24 cases were in Stage I/II and 14 cases Stage III/IV. On the other hand, 16 cases were in low grade, 12 cases intermediate grade, and 10 cases high grade. Twenty of 21 serous carcinomas were strongly positive for WT1, whereas most of the endometrioid carcinomas were negative. Ki67 (MIB1) labeling index and Her2/Neu were higher in both higher grade and stages. On the contrary, the intensity of Bcl2 staining was lower in higher grades and stages lesions.
The use of WT1 may be useful in resolving diagnostic dilemma between serous and endometrioid carcinoma, especially in difficult cases. Ki67, Bcl2, and Her2/Neu may be used as prognostic markers.
卵巢癌是女性癌症死亡的第五大常见原因。它占女性所有癌症的 3%，占生殖系统恶性肿瘤的 15%-20%。大多数卵巢癌为浆液性癌，其次为子宫内膜样癌。有时，这两种癌的腺体在组织学上无法区分。当它们分化不良时，也很难区分这两种类型。
本研究旨在发现浆液性癌和子宫内膜样癌之间免疫标志物表达的差异，以及它们的染色模式与其他临床病理预后因素的关系。
对组织学诊断为 38 例卵巢浆液性和子宫内膜样癌的石蜡包埋组织进行 WT1、B 细胞淋巴瘤 2（Bcl2）、Ki67（MIB1）和 Her2/Neu 的免疫组织化学染色，并将染色模式与其他临床病理预后因素进行相关性分析。
在这 38 例病例中（21 例浆液性癌和 17 例子宫内膜样癌），24 例为Ⅰ/Ⅱ期，14 例为Ⅲ/Ⅳ期。另一方面，16 例为低级别，12 例为中级别，10 例为高级别。21 例浆液性癌中有 20 例 WT1 强阳性，而大多数子宫内膜样癌为阴性。Ki67（MIB1）标记指数和 Her2/Neu 在高级别和分期中更高。相反，高级别和分期病变中 Bcl2 染色强度较低。
WT1 的使用可能有助于解决浆液性癌和子宫内膜样癌之间的诊断难题，特别是在困难的情况下。Ki67、Bcl2 和 Her2/Neu 可作为预后标志物。
