Biotechnology Platform,, Agricultural Research Council, Private Bag X05, Onderstepoort, 0110, South Africa.
Center for Bioinformatics and Computational Biology, Department of Biochemistry, Genetics and Molecular Biology, University of Pretoria, Lynwood Road, Pretoria, 0001, South Africa.
Sci Rep. 2021 Mar 15;11(1):5905. doi: 10.1038/s41598-021-85282-7.
A metagenomic approach was used to study the gut microbiome of Haemonchus contortus field strains and that of its predilection site, the abomasum of Dohne Merino sheep. The abomasum contents and H. contortus were collected from 10 naturally infected Dohne Merino sheep. The H. contortus specimens were classified and sexually differentiated using morphometric characters and was further confirmed through molecular identification. We investigated differences and similarities between the bacterial composition of the adult male and female H. contortus gut microbiomes, which were both dominated by bacteria from the Escherichia, Shigella, Vibrio and Halomonas genera. Major abundance variations were identified between the shared adult male and female H. contortus microbiomes. The results also revealed that Succiniclasticum, Rikenellaceae RC9 gut group and Candidatus Saccharimonas were the predominant genera in the Dohne Merino abomasum. This study provides insight into the highly diverse bacterial composition of the H. contortus gut microbiome and the Dohne Merino abomasum which needs to be studied further to explore the complex interactions of different gastrointestinal nematode microbiomes with the host.
采用宏基因组学方法研究了捻转血矛线虫田间株系和其偏好部位绵羊皱胃的肠道微生物组。从 10 只自然感染的杜罗内美利奴羊中收集皱胃内容物和捻转血矛线虫。通过形态特征对捻转血矛线虫标本进行分类和性别分化，并通过分子鉴定进一步确认。我们研究了雌雄捻转血矛线虫肠道微生物组细菌组成的差异和相似性，两者均以大肠杆菌、志贺氏菌、弧菌和盐单胞菌属的细菌为主。在雌雄共享的捻转血矛线虫微生物组之间鉴定出主要的丰度变化。结果还表明，琥珀酸裂解菌、理研菌科 RC9 肠道群和疑似 Saccharimonas 是杜罗内美利奴羊皱胃的主要属。本研究深入了解了捻转血矛线虫肠道微生物组和杜罗内美利奴羊皱胃高度多样化的细菌组成，需要进一步研究以探索不同胃肠道线虫微生物组与宿主的复杂相互作用。
