Xinjiang Production and Construction Crops Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alaer, 843300, Xinjiang, China.
College of Life Sciences, Tarim University, Alaer, 843300, Xinjiang, China.
J Plant Res. 2021 May;134(3):613-623. doi: 10.1007/s10265-021-01280-w. Epub 2021 Mar 15.
Abiotic stresses such as drought, salinity, and heat affect plant growth and development. Karelinia caspica is a unique perennial herb that grows in desert area for a long time and has strong tolerance to environmental stresses. In order to explore the functions of the Na/H antiporter gene from eremophyte K. caspica (KcNHX1) in the abiotic stress response of K. caspica and the underlying regulatory mechanisms, we constructed a vector overexpressing KcNHX1 and transformed it into Arabidopsis thaliana. The physiological results showed that the overexpression of KcNHX1 in A. thaliana not only enhanced the plant's tolerance to salt stress, but also enhanced its tolerance to drought and heat stress at the seedling stage. In addition, KcNHX1-overexpressing plants exhibited enhanced reproductive growth under high temperature, which was mediated by increased auxin accumulation. Taken together, our results indicate that KcNHX1 from an eremophyte can be used as a candidate gene to improve multiple stress tolerance in other plants.
非生物胁迫如干旱、盐度和热胁迫会影响植物的生长和发育。长柄扁桃是一种独特的多年生草本植物，长期生长在沙漠地区，对环境胁迫具有很强的耐受性。为了探讨来自旱生植物长柄扁桃（KcNHX1）的 Na+/H+逆向转运蛋白基因在长柄扁桃非生物胁迫响应中的功能及其潜在的调控机制，我们构建了一个过表达 KcNHX1 的载体，并将其转化为拟南芥。生理结果表明，在拟南芥中过表达 KcNHX1 不仅增强了植物对盐胁迫的耐受性，而且增强了其在幼苗期对干旱和热胁迫的耐受性。此外，KcNHX1 过表达植株在高温下表现出增强的生殖生长，这是通过增加生长素积累介导的。总之，我们的结果表明，来自旱生植物的 KcNHX1 可以作为候选基因，用于提高其他植物的多种胁迫耐受性。
