转录组芯片筛选和验证口腔白斑癌变相关缺氧反应基因和 microRNA。
Transcriptome array screening and verification of oral leukoplakia carcinogenesis-related hypoxia-responsive gene and microRNA.
机构信息
Dept. of Oral Medicine, Shanghai Ninth People,s Hospital, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China.
Dept. of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People,s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2021 Feb 1;39(1):20-25. doi: 10.7518/hxkq.2021.01.003.
OBJECTIVES
To study the hypoxia response gene and microRNA (miRNA) expression profiles in the pathogenesis and progression of oral leukoplakia (OLK).
METHODS
Affymetrix GeneChip human transcriptome array 2.0 was used to detect the transcriptome of normal mucosa, low-risk OLK, high-risk OLK, and early squamous cell carcinoma (SCC). Gene ontology function analysis was used to screen genes and key miRNAs whose biological role is hypoxia response. Quantitative reverse transcription polymerase ch-ain reaction (qRT-PCR) was used to verify the expression of hypoxia response genes and miRNAs.
RESULTS
A total of 7 different genes of hypoxia response between normal mucosa and low-risk OLK, 10 genes between low-risk and high-risk OLK, and 21 genes between high-risk OLK and SCC were identified. The results of qRT-PCR showed that the expression of hypoxia-inducible factor 1α, chemokine cc-motif ligand 2, and matrix metalloproteinase 3 mRNA and miR-21 in normal mucosa, OLK, and SCC increased in a stepwise manner. The expression difference between OLK and SCC was statistically significant and consistent with the results of transcriptome array.
CONCLUSIONS
The hypoxia response gene and related miRNA play roles in the development and progression of OLK.
目的
研究口腔白斑（OLK）发病机制和进展过程中的缺氧反应基因和 microRNA（miRNA）表达谱。
方法
使用 Affymetrix GeneChip 人类转录组芯片 2.0 检测正常黏膜、低风险 OLK、高风险 OLK 和早期鳞状细胞癌（SCC）的转录组。采用基因本体功能分析筛选具有缺氧反应生物学作用的基因和关键 miRNA。采用定量逆转录聚合酶链反应（qRT-PCR）验证缺氧反应基因和 miRNA 的表达。
结果
在正常黏膜与低风险 OLK、低风险与高风险 OLK、高风险 OLK 与 SCC 之间分别鉴定出 7 个、10 个和 21 个不同的缺氧反应基因。qRT-PCR 结果显示，缺氧诱导因子 1α、趋化因子 cc 基序配体 2 和基质金属蛋白酶 3 mRNA 和 miR-21 在正常黏膜、OLK 和 SCC 中的表达呈逐步增加。OLK 和 SCC 之间的表达差异具有统计学意义，与转录组阵列的结果一致。
结论
缺氧反应基因和相关 miRNA 在 OLK 的发生发展中起作用。
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