建立贝类中诺如病毒的逆转录（RT）聚合酶链反应（PCR）检测方法。
Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs.
机构信息
QC Laboratory, Post Harvest Technology, ICAR- Central Institute of Fisheries Education (CIFE), Mumbai, 400061, India E-mail:
Aquatic Environment & Health Management Division, ICAR- Central Institute of Fisheries Education (CIFE), Mumbai, 400061, India.
出版信息
Water Sci Technol. 2021 Mar;83(5):1103-1107. doi: 10.2166/wst.2021.048.
Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on the RNA-dependent RNA polymerase gene of norovirus genogroup II (NoV GII). Samples of bivalves were processed for the concentration of virus and extraction of RNA, followed by reverse transcription PCR. A total of 50 molluscan shellfish samples were analyzed, of which 16 samples yielded positive amplifications of norovirus nucleic acid. The PCR method described here, involving a single set of primers, is useful for rapid screening of shellfish for NoV GII.
诺如病毒是重要的食源性致病菌，常与滤食性双壳贝类的食用有关。在此，我们报告了一种基于诺如病毒基因 II 组（NoV GII）的 RNA 依赖性 RNA 聚合酶基因的逆转录聚合酶链反应（RT-PCR）方法的开发。对贝类样本进行病毒浓缩和 RNA 提取，然后进行逆转录 PCR。共分析了 50 个贝类样本，其中 16 个样本检测到诺如病毒核酸阳性。本文描述的 PCR 方法，使用了一套引物，可用于快速筛选贝类中的 NoV GII。
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