Guilan Road Trauma Research Center, Guilan University of Medical Science, Rasht, Iran.
Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Psychiatr Res. 2021 May;137:260-265. doi: 10.1016/j.jpsychires.2021.02.045. Epub 2021 Mar 9.
Cannabis and ecstasy are illicit substances, and currently, there are no approved treatments for methamphetamine (METH) use disorder. Some studies have proposed that cannabidiol (CBD) decreases the motivation for METH seeking, but reports indicate that the therapeutic benefits are only for heroin. Here, we studied the interaction between CBD and METH during the sensitization phase on the rewarding effect of METH, using the conditioned place preference (CPP) paradigm to measure possible alterations in sensitivity. Our data showed that i. p. injection of METH created METH-induced CPP at two of the highest applied doses (1 and 2 mg/kg), and injection of METH during the sensitization period caused an establishment of METH-induced CPP at lower doses (0.25 and 0.5 mg/kg). Data also revealed that i. c.v. administration of CBD during the sensitization phase, shifted the establishment of METH-induced CPP toward a lower dose (0.5 mg/kg). Concurrent administration of CBD (10 μg/5 μl, i. c.v.) and METH (0.25 mg/kg, i. p.) during sensitization phase established METH-induced CPP with sub-threshold doses of METH (0.125, 0.25, and 0.5 mg/kg). Our results suggest the involvement of CBD and prior exposure to METH in creating sensitization to METH CPP.
大麻和摇头丸是非法物质，目前尚无批准的治疗甲基苯丙胺（METH）使用障碍的方法。一些研究提出大麻二酚（CBD）可以降低对 METH 的寻求动机，但报告表明其治疗益处仅针对海洛因。在这里，我们使用条件位置偏好（CPP）范式研究了 CBD 和 METH 在敏化阶段对 METH 奖赏效应的相互作用，以测量对敏感性的可能改变。我们的数据表明，i.p. 注射 METH 在两种最高应用剂量（1 和 2mg/kg）下产生 METH 诱导的 CPP，并且在敏化期间注射 METH 导致在较低剂量（0.25 和 0.5mg/kg）下建立 METH 诱导的 CPP。数据还表明，在敏化阶段 i.c.v. 给予 CBD 会使 METH 诱导的 CPP 向较低剂量（0.5mg/kg）转移。在敏化阶段同时给予 CBD（10μg/5μl，i.c.v.）和 METH（0.25mg/kg，i.p.），用亚阈值剂量的 METH（0.125、0.25 和 0.5mg/kg）建立 METH 诱导的 CPP。我们的结果表明 CBD 的参与和先前暴露于 METH 会导致对 METH CPP 的敏化。
