Wexler M, Gordon D M, Murphy P J
Department of Crop Protection, University of Adelaide, Glen Osmond, SA 5064, Australia.
Division of Botany and Zoology, Australian National University, Canberra, ACT 0200, Australia.
Microbiology (Reading). 1996 May;142(5):1059-1066. doi: 10.1099/13500872-142-5-1059.
Chromosomal and symbiosis-related genotypes of rhizopine-producing and non-producing isolates of and were examined by multilocus enzyme electrophoresis and RFLP. The distribution of rhizopine production in both species was found to be independent of host genotype. Conversely, rhizopine production was associated with particular symbiotic plasmid types. This association may explain the observed distribution of rhizopine production in and . Rhizopine synthesis () genes showed greater sequence divergence than rhizopine catabolism () genes in both and . Furthermore, and genes were less divergent in than , suggesting a more recent evolution in the former species.
通过多位点酶电泳和限制性片段长度多态性（RFLP）分析了发根农杆菌（Agrobacterium rhizogenes）产根瘤碱和不产根瘤碱分离株的染色体及共生相关基因型。发现这两个物种中根瘤碱产生的分布与宿主基因型无关。相反，根瘤碱的产生与特定的共生质粒类型有关。这种关联可能解释了在发根农杆菌和根癌农杆菌（Agrobacterium tumefaciens）中观察到的根瘤碱产生的分布情况。在发根农杆菌和根癌农杆菌中，根瘤碱合成（rol）基因的序列差异比根瘤碱分解代谢（roa）基因更大。此外，发根农杆菌中的rol和roa基因的差异比根癌农杆菌中的小，这表明前者物种的进化时间更近。
