Kim Chang-Soo, Kim Junyoung, Yoo Hongki
Opt Express. 2021 Mar 1;29(5):6509-6522. doi: 10.1364/OE.416999.
Reflectance confocal microscopy is widely used for non-destructive optical three-dimensional (3D) imaging. In confocal microscopy, a stack of sequential two-dimensional (2D) images with respect to the axial position is typically needed to reconstruct a 3D image. As a result, in conventional confocal microscopy, acquisition speed is often limited by the rate of mechanical scanning in both the transverse and axial directions. We previously reported a high-speed parallel confocal detection method using a pinhole array for color 3D imaging without any mechanical scanners. Here, we report a high-speed color 3D imaging method based on patterned illumination employing a negative pinhole array, whose optical characteristics are the reverse of the conventional pinhole array for transmitting light. The negative pinhole array solves the inherent limitation of a conventional pinhole array, i.e., low transmittance, meaning brighter color images with abundant color information can be acquired. We also propose a 3D image processing algorithm based on the 2D cross-correlation between the acquired image and filtering masks, to produce an axial response. By using four-different filtering masks, we were able to increase the sampling points in calculation of height and enhance the lateral resolution of the color acquisition by a factor of four. The feasibility of high-speed non-contact color 3D measurement with the improved lateral resolution and brightness provided by the negative pinhole array was demonstrated by imaging various specimens. We anticipate that this high-speed color 3D measurement technology with negative pinhole array will be a useful tool in a variety of fields where rapid and accurate non-contact measurement are required, such as industrial inspection and dental scanning.
反射共聚焦显微镜广泛用于无损光学三维(3D)成像。在共聚焦显微镜中,通常需要一系列关于轴向位置的二维(2D)图像堆栈来重建3D图像。因此,在传统的共聚焦显微镜中,采集速度常常受到横向和轴向机械扫描速率的限制。我们之前报道了一种使用针孔阵列进行彩色3D成像的高速并行共聚焦检测方法,无需任何机械扫描仪。在此,我们报道一种基于图案照明的高速彩色3D成像方法,该方法采用负针孔阵列,其光学特性与传统的用于透光的针孔阵列相反。负针孔阵列解决了传统针孔阵列固有的局限性,即低透射率,这意味着可以获取具有丰富颜色信息的更明亮彩色图像。我们还提出了一种基于采集图像与滤波掩模之间二维互相关的3D图像处理算法,以产生轴向响应。通过使用四种不同的滤波掩模,我们能够在高度计算中增加采样点,并将彩色采集的横向分辨率提高四倍。通过对各种样本成像,证明了利用负针孔阵列提供的更高横向分辨率和亮度进行高速非接触式彩色3D测量的可行性。我们预计,这种具有负针孔阵列的高速彩色3D测量技术将成为在各种需要快速准确非接触测量的领域,如工业检测和牙科扫描中的有用工具。