Hirota N, Furuta T, Kasuya Y
Tokyo College of Pharmacy, Japan.
J Chromatogr. 1988 Mar 18;425(2):237-43. doi: 10.1016/0378-4347(88)80028-8.
A capillary gas chromatographic-mass spectrometric method for the determination of cortisol in human plasma using cortisol M + 5 as an internal standard is described. For calculation of plasma cortisol, peak areas were measured by selected-ion monitoring of the characteristic fragment ions of the dimethoxime-tri (trimethylsilyl) derivatives of cortisol and cortisol M + 5 (m/z 605 and 610, respectively). The inter- and intra-assay coefficients of variation for plasma sample were 3.07 and 1.77%, respectively. The method needed no complex corrections for contributions and provides a sensitive and reliable technique with good accuracy, precision and reproducibility.
描述了一种毛细管气相色谱 - 质谱法，该方法以皮质醇M + 5作为内标物来测定人血浆中的皮质醇。为了计算血浆皮质醇，通过对皮质醇及其M + 5的二甲肟 - 三（三甲基硅基）衍生物的特征碎片离子（分别为m/z 605和610）进行选择离子监测来测量峰面积。血浆样品的批间和批内变异系数分别为3.07%和1.77%。该方法无需对贡献进行复杂校正，提供了一种具有良好准确性、精密度和重现性的灵敏且可靠的技术。
