在手性固定相上通过高效液相色谱法分离脂肪酸酯和酰胺对映体。
Separation of fatty acid ester and amide enantiomers by high-performance liquid chromatography on chiral stationary phases.
作者信息
Weaner L E, Hoerr D C
机构信息
Department of Chemical Development, McNeil Pharmaceutical, Spring House, PA 19477-0776.
出版信息
J Chromatogr. 1988 Mar 11;437(1):109-19. doi: 10.1016/s0021-9673(00)90374-1.
The enantiomeric resolution of a series of chiral fatty acid epoxide and alpha-substituted palmitic acid analogues was examined by high-performance liquid chromatography on chiral stationary phases. The compounds were chromatographed as ester or amide derivatives on commercially available stationary phases that consisted of (R)-N-(3,5-dinitrobenzoyl)phenylglycine either covalently or ionically bonded to aminopropylsilica gel. Factors affecting separation included hydrocarbon chain length of the fatty acid, the type of substituents attached to the chiral center, the type of derivative, and column temperature. Effects of sample size, mobile phase composition, column type, and flow-rate on the resolution and separation factor values were also explored.
通过在手性固定相上进行高效液相色谱法，对一系列手性脂肪酸环氧化物和α-取代棕榈酸类似物进行了对映体拆分。这些化合物以酯或酰胺衍生物的形式在市售固定相上进行色谱分析，该固定相由共价或离子键合到氨丙基硅胶上的(R)-N-(3,5-二硝基苯甲酰基)苯甘氨酸组成。影响分离的因素包括脂肪酸的烃链长度、连接到手性中心的取代基类型、衍生物类型和柱温。还探讨了进样量、流动相组成、柱类型和流速对分离度和分离因子值的影响。
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