Laboratory of Morphophysiology and Animal Health, Amapá State University. Presidente Vargas Avenue, 650, downtown, Macapá, AP 68900-070, Brazil.
Carlos Azevedo Research Laboratory, Federal Rural University of the Amazon (UFRA), Presidente Tancredo Neves Avenue, 2501, Montese, Belém 66077-901, Pará, Brazil.
Parasitol Int. 2021 Aug;83:102332. doi: 10.1016/j.parint.2021.102332. Epub 2021 Mar 13.
The present study describes a new coelozoic, eukaryotic microparasite of the genus Ellipsomyxa Køie, 2003 (Ceratomyxidae: Myxozoa) found parasitizing the gallbladder of Satanoperca jurupari Heckel, 1840 collected in the Curiaú River Environmental Protection Area in Macapá, Amapá state, Brazil. The fish were collected using mesh cast net. The gallbladders were examined, preserved in 80% alcohol for molecular analysis (SSU rDNA gene), and fixed in Davidson for histological slide preparation. The new parasite had a prevalence of 81% in the gallbladder, asymmetric plasmodia, irregular free spores in the bladder fluid, with no cyst formation. The spores are elliptical, with characteristics of the genus Ellipsomyxa, and they had a mean length of 10.11 (8.56-10.5) μm, mean width of 7.81 (5.96-9.56) μm, and thick walls. The polar capsules are sub-spherical in shape, slightly asymmetrical, with a mean length of 3.12 (2.31-3.99) μm and mean width of 2.5 (2.22-2.95) μm, containing polar filament with five or six coils perpendicular to the longitudinal axis of the capsule. The Bayesian Inference assigned the new species to a subclade formed by a lineage of Ellipsomyxa species from the Amazon region. Ellipsomyxa tucujuensis n. sp. is the sixth species of this genus described in fish from the Amazon region, and the first for the state of Amapá.
本研究描述了一种新的内生性真核微寄生原虫，属于科（Myxozoa）的 Ellipsomyxa Køie, 2003 属（Ceratomyxidae），寄生在巴西阿马帕州库里亚乌河自然保护区采集的撒旦魔鱼（Satanoperca jurupari Heckel, 1840）的胆囊中。这些鱼是用网兜捕捞的。检查胆囊，用 80%酒精保存用于分子分析（SSU rDNA 基因），并固定在 Davidson 中用于制作组织学幻灯片。新寄生虫在胆囊中的流行率为 81%，有不对称的原生质体，膀胱液中有不规则的自由孢子，没有囊形成。孢子呈椭圆形，具有 Ellipsomyxa 属的特征，平均长度为 10.11μm（8.56-10.5μm），平均宽度为 7.81μm（5.96-9.56μm），壁厚。极囊呈近球形，略不对称，平均长度为 3.12μm（2.31-3.99μm），平均宽度为 2.5μm（2.22-2.95μm），含有极丝，极丝与胶囊的纵轴垂直，有五或六个螺旋。贝叶斯推断将新种分配到一个亚分支，该分支由亚马逊地区的 Ellipsomyxa 物种组成。Ellipsomyxa tucujuensis n. sp. 是亚马逊地区鱼类中描述的第六种该属的物种，也是阿马帕州的第一种。
