Surgical Department, University Hospital Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Institute of Pathology, University Hospital Mannheim, Heidelberg University, Mannheim, Germany.
Sci Rep. 2021 Mar 16;11(1):6030. doi: 10.1038/s41598-021-85400-5.
Carcinosarcoma of the urinary bladder is a very rare and aggressive subtype of bladder cancer with poor prognosis. Characteristically carcinosarcomas exhibit biphasic nature with both epithelial and mesenchymal differentiation. Limited information is available regarding its clinical features and appropriate treatments due to its rarity. Development of tumour models can further our understanding of bladder carcinosarcoma. We report establishment and characterization of the first-ever bladder carcinosarcoma cell line MaS-3. It is established by the outgrow method from 86 year-old caucasian male who underwent a radical pelvic resection after neoadjuvant radiotherapy. MaS-3 showed carcinosarcoma profile with high conformity with to the original tumour in terms of immunocytochemistry. Proteome analysis also aligned the MaS-3 cell line with the carcinosarcoma specimen rather than corresponding non-malignant tissue. Chemotherapy sensitivity testing revealed a great sensitivity of MaS-3 growth to 5-Fluorouracil, Gemcitabine and Cisplatin, with almost no impact of Irinotecan. Additionally, the suitability of MaS-3 for 3D in vitro experiments was also demonstrated. The newly established cell line MaS-3 shows typical characteristics of the tumour and may thus be a useful in vitro model system for studying the tumour biology and developing future of treatments of this rare but very aggressive entity.
膀胱癌癌肉瘤是一种非常罕见且侵袭性强的膀胱癌亚型，预后较差。癌肉瘤的特征是具有上皮和间叶分化的双相性。由于其罕见性，关于其临床特征和适当治疗的信息有限。肿瘤模型的发展可以进一步加深我们对膀胱癌肉瘤的理解。我们报告了首例膀胱癌癌肉瘤细胞系 MaS-3 的建立和鉴定。它是通过从一位 86 岁的白人男性的体外培养方法建立的，该男性在新辅助放疗后接受了根治性盆腔切除术。MaS-3 表现出癌肉瘤特征，在免疫细胞化学方面与原始肿瘤高度一致。蛋白质组分析也表明 MaS-3 细胞系与癌肉瘤标本一致，而与相应的非恶性组织不一致。化疗敏感性测试显示 MaS-3 对 5-氟尿嘧啶、吉西他滨和顺铂的生长非常敏感，而伊立替康几乎没有影响。此外，还证明了 MaS-3 适合 3D 体外实验。新建立的 MaS-3 细胞系表现出肿瘤的典型特征，因此可能是研究肿瘤生物学和开发这种罕见但非常侵袭性实体的未来治疗方法的有用体外模型系统。
