Antonov A S, Bakalkin G Ia, Krivoshev O G, Isachenkov V A
Probl Endokrinol (Mosk). 1977 Sep-Oct;23(5):51-5.
The presence of immunoreactive LH-releasing hormone (LH-RH) was revealed in the epiphysis of rats by radioimmunochemical method. The content of this hormone displayed circadian variations: it was maximum at 6 p.m. and minimum at 6 a.m. Intravenous injection of synthetic LG-RH to infantile and sexually mature rats of both sexes induced in the epiphysis the activity of hydroxyindol-O-methyl transpherase (HIOMT), the key enzyme of melatonin synthesis. Increased activity of this enzyme in the epiphysis persisted for at least 5 hours after the administration of LH-RH. HIOMT activation is preceded by a rapid fall (in 5 minutes) of cyclic adenosine-3', 5'-monophosphate in the epiphysis. The data obtained permitted to suppose that feed-back of the hypothalamus with the epiphysis could be realized with the aid of LH-RH.
用放射免疫化学方法在大鼠松果体中发现了免疫反应性促黄体生成素释放激素（LH-RH）。这种激素的含量呈现昼夜变化：下午6点时最高，上午6点时最低。给幼年和性成熟的雌雄大鼠静脉注射合成的LH-RH，可诱导松果体中褪黑素合成的关键酶——羟基吲哚-O-甲基转移酶（HIOMT）的活性。在注射LH-RH后，松果体中该酶活性的增加持续至少5小时。HIOMT的激活之前，松果体中的环磷酸腺苷（cAMP）会迅速下降（5分钟内）。所获得的数据表明，下丘脑与松果体之间的反馈可能借助LH-RH来实现。
