Department of Oncology, Shenzhen Baoan Hospital of Traditional Chinese Medicine, Shenzhen, China.
Department of Breast Surgery, The Second People's Hospital of Shenzhen, Shenzhen, China.
Kaohsiung J Med Sci. 2021 Jul;37(7):572-582. doi: 10.1002/kjm2.12371. Epub 2021 Mar 17.
Nobiletin is a polymethoxylated flavone present in citrus fruits, which has been reported to have inhibitory effects on tumorigenesis of cancers. However, the biological function of nobiletin in breast cancer (BC) is largely unknown. To investigate the effect of nobiletin on growth of BC cells, the cell viability of BC was measured by MTT assay. In addition, gene and protein expressions were detected by qRT-PCR and western blot, respectively. The apoptosis and pyroptosis of BC cells were tested by flow cytometry. Finally, the correlation between miR-200b and JAZF1 was detected by dual luciferase report. The data indicated that nobiletin inhibited the proliferation of BC cells in a dose-dependent manner. Moreover, miR-200b mimics-induced pyroptosis of BC cells was further increased by nobiletin. Meanwhile, JAZF1 was found to be the target of miR-200b. Moreover, nobiletin induced apoptosis and pyroptosis of BC cells via miR-200b/JAZF1/NF-κB axis. In conclusion, nobiletin inhibited the tumorigenesis of BC via regulation of miR-200b/JAZF1 axis. Thus, nobiletin might serve as a new agent for the treatment of BC.
川陈皮素是柑橘类水果中一种多甲氧基黄酮，已有报道称其对癌症的肿瘤发生具有抑制作用。然而，川陈皮素在乳腺癌（BC）中的生物学功能在很大程度上尚不清楚。为了研究川陈皮素对 BC 细胞生长的影响，通过 MTT 测定法测量了 BC 的细胞活力。此外，通过 qRT-PCR 和 Western blot 分别检测了基因和蛋白质的表达。通过流式细胞术测试了 BC 细胞的凋亡和细胞焦亡。最后，通过双荧光素酶报告检测了 miR-200b 和 JAZF1 之间的相关性。数据表明，川陈皮素呈剂量依赖性抑制 BC 细胞的增殖。此外，川陈皮素进一步增加了 miR-200b 模拟物诱导的 BC 细胞细胞焦亡。同时，发现 JAZF1 是 miR-200b 的靶标。此外，川陈皮素通过 miR-200b/JAZF1/NF-κB 轴诱导 BC 细胞凋亡和细胞焦亡。总之，川陈皮素通过调节 miR-200b/JAZF1 轴抑制 BC 的肿瘤发生。因此，川陈皮素可能成为治疗 BC 的一种新药物。
