School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Nat Prod Res. 2022 May;36(10):2554-2558. doi: 10.1080/14786419.2021.1900176. Epub 2021 Mar 17.
Bailemian capsule (BLMC) is a Chinese patent drug for treating insomnia with excellent curative effects. But there are few researches on it. In this research, a rapid separation and identification method using UPLC-QE-Orbitrap-MS was established, and 228 identified compounds were separated within 18 min. The structures of compounds were preliminarily determined by comparing the retention time and fragmentation law. Furthermore, multiple databases were used to integrate the compound targets of BLMC and the disease targets related to insomnia. After the intersection of the two sets of targets, a protein-protein interaction network and a drug-target-disease pharmacological network were established, then using the DAVID database to perform GO analysis and KEGG analysis on the common targets to find related pathways. Finally, a total of 289 common targets and 136 pathways were found to participate in the mechanism.
眠安宁胶囊是一种治疗失眠的中药专利药，疗效确切。但目前关于眠安宁胶囊的研究较少。本研究建立了一种基于 UPLC-QE-Orbitrap-MS 的快速分离鉴定方法，可在 18 min 内分离出 228 种鉴定化合物。通过比较保留时间和裂解规律初步确定了化合物的结构。进一步将眠安宁胶囊的化合物靶标与失眠相关疾病靶标进行整合，通过多个数据库的交集，构建了蛋白-蛋白相互作用网络和药物-靶标-疾病药理学网络，然后对共同靶标进行 DAVID 数据库的 GO 分析和 KEGG 分析，寻找相关通路。最终共发现 289 个共同靶标和 136 条通路参与其作用机制。
