Galindo-Hernandez Octavio, Leija-Montoya Ana Gabriela, Romero-Garcia Tatiana, Vazquez-Jimenez Jose Gustavo
Autonomous University of Baja California, Laboratory of Biochemistry, School of Medicine, Campus Mexicali, BC, Mexico.
Autonomous University of Baja California, Laboratory of Biochemistry, Sports School, Campus Mexicali, BC, Mexico.
Genet Mol Biol. 2021 Mar 15;44(1):e20200279. doi: 10.1590/1678-4685-GMB-2020-0279. eCollection 2021.
Palmitic acid, the main saturated fatty acid, is related with a wide range of metabolic disorders such as obesity, type 2 diabetes and heart disease. It is known that palmitic acid disturbs the expression of some important proteins for cell homeostasis such as SERCA and RGS2, however, the role of this lipid at the molecular level in these disorders is not completely elucidated. Thus, our aim was to determinate the effect of palmitic acid in a relevant cell process as it is cell migration and the participation of SERCA and RGS2 in this response. We found that palmitic acid reduces cell migration (determined by the Boyden chamber method) in an epithelial cell line (HEK293) and this effect is modulated by SERCA and RGS2 differential protein expression (measured by western blot). Also, overexpression of individual proteins, RGS2 and SERCA, produced a decrease and an increase on cell migration, respectively. Taken together, these data suggest that the expression of regulatory proteins is affected by high concentrations of saturated fatty acids and in consequence cell migration is diminished in epithelial cells.
棕榈酸作为主要的饱和脂肪酸，与多种代谢紊乱相关，如肥胖症、2型糖尿病和心脏病。已知棕榈酸会干扰一些维持细胞稳态的重要蛋白质的表达，如肌浆网钙ATP酶（SERCA）和G蛋白信号调节因子2（RGS2），然而，这种脂质在这些疾病分子水平上的作用尚未完全阐明。因此，我们的目的是确定棕榈酸在细胞迁移这一相关细胞过程中的作用，以及SERCA和RGS2在该反应中的参与情况。我们发现，棕榈酸会降低上皮细胞系（HEK293）的细胞迁移能力（通过博伊登小室法测定），且这种作用受SERCA和RGS2差异蛋白表达的调节（通过蛋白质印迹法测定）。此外，单独过表达RGS2和SERCA蛋白，分别会导致细胞迁移能力下降和上升。综上所述，这些数据表明，调节蛋白的表达受高浓度饱和脂肪酸影响，进而上皮细胞的迁移能力减弱。
