肿瘤 miRNA 同型物百科全书（TIE）：一个 miRNA 同型物的泛癌数据库。
Tumor IsomiR Encyclopedia (TIE): a pan-cancer database of miRNA isoforms.
机构信息
RNA Mediated Gene Regulation Section, RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.
Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
出版信息
Bioinformatics. 2021 Sep 29;37(18):3023-3025. doi: 10.1093/bioinformatics/btab172.
SUMMARY
MicroRNAs (miRNAs) are master regulators of gene expression in cancers. Their sequence variants or isoforms (isomiRs) are highly abundant and possess unique functions. Given their short sequence length and high heterogeneity, mapping isomiRs can be challenging; without adequate depth and data aggregation, low frequency events are often disregarded. To address these challenges, we present the Tumor IsomiR Encyclopedia (TIE): a dynamic database of isomiRs from over 10 000 adult and pediatric tumor samples in The Cancer Genome Atlas (TCGA) and The Therapeutically Applicable Research to Generate Effective Treatments (TARGET) projects. A key novelty of TIE is its ability to annotate heterogeneous isomiR sequences and aggregate the variants obtained across all datasets. Results can be browsed online or downloaded as spreadsheets. Here, we show analysis of isomiRs of miR-21 and miR-30a to demonstrate the utility of TIE.
AVAILABILITY AND IMPLEMENTATION
TIE search engine and data are freely available to use at https://isomir.ccr.cancer.gov/.
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
摘要
MicroRNAs（miRNAs）是癌症中基因表达的主要调控因子。它们的序列变体或同工型（isomiRs）非常丰富，具有独特的功能。鉴于它们的短序列长度和高度异质性，对 isomiRs 的映射可能具有挑战性；如果没有足够的深度和数据聚合，低频事件通常会被忽略。为了解决这些挑战，我们提出了肿瘤 isomiR 百科全书（TIE）：一个来自癌症基因组图谱（TCGA）和治疗应用研究以产生有效治疗（TARGET）项目中超过 10000 个成人和儿科肿瘤样本的 isomiRs 的动态数据库。TIE 的一个关键新颖之处在于它能够注释异构的 isomiR 序列，并在所有数据集之间聚合获得的变体。结果可以在线浏览或下载为电子表格。在这里，我们展示了 miR-21 和 miR-30a 的 isomiRs 分析，以展示 TIE 的实用性。
可用性和实现
TIE 搜索引擎和数据可在 https://isomir.ccr.cancer.gov/ 免费使用。
补充信息
补充数据可在生物信息学在线获得。
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