加纳沃尔特地区结核分枝杆菌复合群的分子流行病学。
Molecular epidemiology of Mycobacterium tuberculosis complex in the Volta Region of Ghana.
机构信息
Bacteriology Department, Noguchi Memorial Institute for Medical Research (NMIMR), University of Ghana, Accra, Ghana.
West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana.
出版信息
PLoS One. 2021 Mar 17;16(3):e0238898. doi: 10.1371/journal.pone.0238898. eCollection 2021.
CONTEXT
Available molecular epidemiological data from recent studies suggest significant genetic variation between the different lineages of Mycobacterium tuberculosis complex (MTBC) and the MTBC lineages might have adapted to different human populations.
AIM
This study sought to determine the population structure of clinical MTBC isolates from the Volta Region of Ghana.
METHODS
The MTBC isolates obtained from collected sputum samples were identified by PCR detecting of IS6110 and genotyped using spoligotyping. Non-tuberculous mycobacterial isolates were characterized by amplification of the heat shock protein 65 (hsp65) gene and sequencing. The drug susceptibility profiles of the MTBCs determined using GenoType MTBDRplus.
RESULTS
One hundred and seventeen (117, 93.6%) out of 125 mycobacterial positive isolates were characterized as members of the MTBC of which M. tuberculosis sensu stricto (MTBss) and M. africanum (MAF) were respectively 94 (80.3%) and 23 (19.7%). In all, 39 distinct spoligotype patterns were obtained; 26 for MTBss and 13 for MAF lineages. Spoligotyping identified 89 (76%) Lineage 4, 16 (13.6%) Lineage 5, 7 (6.0%) Lineage 6, 3 (2.6%) Lineage 2, 1(0.9%) Lineage 3 and 1 (0.9%) Lineage 1. Among the Lineage 4 isolates, 62/89 (69.7%) belonged to Cameroon sub-lineage, 13 (14.7%) Ghana, 8 (9.0%) Haarlem, 2 (2.2%) LAM, 1 (1.1%) Uganda I, 1 (1.1%) X and the remaining two (2.2%) were orphan. Significant localization of MAF was found within the Ho municipality (n = 13, 29.5%) compared to the more cosmopolitan Ketu-South/Aflao (n = 3, 8.3%) (p-value = 0.017). Eight (8) non-tuberculous mycobacteria were characterized as M. abscessus (7) and M. fortuitum (1).
CONCLUSION
We confirmed the importance of M. africanum lineages as a cause of TB in the Volta region of Ghana.
背景
最近的研究提供了分子流行病学数据，表明结核分枝杆菌复合群（MTBC）的不同谱系之间存在显著的遗传变异，并且 MTBC 谱系可能已经适应了不同的人群。
目的
本研究旨在确定加纳沃尔特地区临床 MTBC 分离株的种群结构。
方法
从收集的痰样本中获得的 MTBC 分离株通过 PCR 检测 IS6110 进行鉴定，并通过 spoligotyping 进行基因分型。非结核分枝杆菌分离株通过扩增热休克蛋白 65（hsp65）基因并测序进行鉴定。MTBC 的药敏谱使用 GenoType MTBDRplus 确定。
结果
在 125 例分枝杆菌阳性分离株中，有 117 株（93.6%）被鉴定为 MTBC 成员，其中结核分枝杆菌（MTBss）和非洲分枝杆菌（MAF）分别为 94 株（80.3%）和 23 株（19.7%）。总共获得了 39 种不同的 spoligotype 模式；26 种用于 MTBss，13 种用于 MAF 谱系。 spoligotyping 鉴定出 89 株（76%）谱系 4、16 株（13.6%）谱系 5、7 株（6.0%）谱系 6、3 株（2.6%）谱系 2、1 株（0.9%）谱系 3 和 1 株（0.9%）谱系 1。在谱系 4 分离株中，62/89（69.7%）属于喀麦隆亚谱系，13/89（14.7%）属于加纳，8/89（9.0%）属于哈雷姆，2/89（2.2%）属于 LAM，1/89（1.1%）属于乌干达 I，1/89（1.1%）属于 X，其余 2/89（2.2%）属于孤儿。与更具世界性的 Ketu-South/Aflao（n=3，8.3%）相比，霍市（n=13，29.5%）发现了大量的 MAF（p 值=0.017）。8 株非结核分枝杆菌被鉴定为 M. abscessus（7 株）和 M. fortuitum（1 株）。
结论
我们证实了非洲分枝杆菌谱系作为加纳沃尔特地区结核病病因的重要性。
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