Yamada K, Larsson B S, Roberto A, Dencker L, Ullberg S
Department of Toxicology, Uppsala Biomedical Center, Uppsala University, Sweden.
J Invest Dermatol. 1988 Jun;90(6):873-6. doi: 10.1111/1523-1747.ep12462131.
The uptake and retention of 14C-thiouracil and 125I-thiouracil in small lung metastases of B16 murine melanoma was studied in beige mice injected intravenously with melanoma cells. By impulse counting of excised tumor and organ pieces, a high concentration of radioactivity was found in the lung metastases, as compared to normal tissues. The highest tumor/organ concentration ratios appeared 24 h after injection of the radiolabeled thiouracil. A separate autoradiographic study on the disposition of 14C-thiouracil in mice with melanoma metastases confirmed the impulse counting results and also showed the absence of any other site of retention of radioactivity except for hair follicles and to some extent the thyroid. The selective uptake of 14C- and 125I-thiouracil in melanomas depends on their acceptance as false melanin precursors, making them specific markers for growing melanin. The results indicate that radiolabeled thiouracil may be useful for clinical diagnosis and, possibly, therapy of malignant melanotic melanomas.
在给米色小鼠静脉注射黑色素瘤细胞后，研究了14C - 硫脲嘧啶和125I - 硫脲嘧啶在B16小鼠黑色素瘤小肺转移灶中的摄取和滞留情况。通过对切除的肿瘤和器官组织块进行脉冲计数，发现与正常组织相比，肺转移灶中放射性浓度很高。注射放射性标记的硫脲嘧啶后24小时出现最高的肿瘤/器官浓度比。一项关于14C - 硫脲嘧啶在患有黑色素瘤转移的小鼠体内分布的单独放射自显影研究证实了脉冲计数结果，并且还表明除毛囊以及在一定程度上甲状腺外，不存在任何其他放射性滞留部位。14C - 和125I - 硫脲嘧啶在黑色素瘤中的选择性摄取取决于它们被接受为假黑色素前体，使其成为生长中的黑色素的特异性标记物。结果表明，放射性标记的硫脲嘧啶可能对恶性黑色素性黑色素瘤的临床诊断以及可能的治疗有用。
