Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
Department of Medical-Surgical Nursing, College of Nursing, King Saud University, Riyadh, Saudi Arabia.
J Reprod Immunol. 2021 Jun;145:103310. doi: 10.1016/j.jri.2021.103310. Epub 2021 Mar 10.
A lot of research investigations, conducted in the recent years, establishes that microRNAs (miRNAs) have an important role in keeping both growth and metastasis of Ovarian Epithelial Carcinoma (OEC) under control. However, the clinical and functional role of miR-130a-3p in OEC is yet to be explored. Through quantitative reaction i.e., qRT-PCR, the expression of miR-130a-3p was assessed in tissues and cell lines of OEC patients. This analysis determined the relationship between the expression of miR-130a-3p and its clinicopathology with the overall survival rate of OEC patients. The author made use of cell counting analysis (CCK8) and in vitro flow cytometry to understand the functional and biological impact of miR-130a-3p expression. In comparison with neighboring normal tissues, the expression of miR-130a-3p was found to be lower in 7 OEC samples. Few reasons are cited for this scenario i.e., low expression of miR-130a-3p, such as low overall OEC patient survival rate, incidence of FIGO and metastasis of lymph nodes. miR-130a-3p has been found as an independent candidate for predicting the prognosis of OEC patients, as per Multivariate Cox research. When miR-130a-3p is over-expressed, as per the enhanced mechanism, it prevents both cell proliferation and cell cycle production in OEC. The current study findings emphasize that miR-130a-3p can be leveraged as a biomarker of prognosis and possibly as a target in the treatment of OEC.
近年来，大量研究表明，微小 RNA（miRNAs）在控制卵巢上皮性癌（OEC）的生长和转移方面起着重要作用。然而，miR-130a-3p 在 OEC 中的临床和功能作用仍有待探索。通过定量反应，即 qRT-PCR，评估了 OEC 患者组织和细胞系中 miR-130a-3p 的表达。该分析确定了 miR-130a-3p 的表达与其临床病理学与 OEC 患者总生存率之间的关系。作者利用细胞计数分析（CCK8）和体外流式细胞术来了解 miR-130a-3p 表达的功能和生物学影响。与邻近的正常组织相比，在 7 个 OEC 样本中发现 miR-130a-3p 的表达较低。造成这种情况的原因有几个，例如 miR-130a-3p 表达低，总 OEC 患者生存率低，FIGO 发生率和淋巴结转移。根据多变量 Cox 研究，miR-130a-3p 被认为是预测 OEC 患者预后的独立候选者。根据增强机制，当 miR-130a-3p 过表达时，它可以防止 OEC 中的细胞增殖和细胞周期产生。本研究结果强调，miR-130a-3p 可以作为预后的生物标志物，并可能作为 OEC 治疗的靶点。
