Selvarajan Sandhiya, Srinivasan Akila, Surendran Deepanjali, Mathaiyan Jayanthi, Kamalanathan Sadishkumar
Department of Clinical Pharmacology, JIPMER, Puducherry, India.
Department of Medicine, JIPMER, Puducherry, India.
Drug Metab Pers Ther. 2021 Mar 17. doi: 10.1515/dmdi-2020-0178.
To evaluate the association of polymorphisms (, and ) with vitamin D levels and glycemic status in type 2 diabetes patients from Southern India.
In this observational study, genotype frequencies and vitamin D levels of 200 cases (type 2 diabetes patients) were compared with 300 controls (unrelated anonymised stored samples of healthy volunteers) from south India. Serum 25 (OH) D levels were measured by immunoassay technique, glycated hemoglobin (HbA) was measured using HPLC and genotyping of VDR polymorphisms were carried out using Real time Polymerase Chain Reaction (RT PCR).
About 69.2% of type 2 diabetes patients were found to have vitamin D deficiency. polymorphism showed variations in serum 25 (OH) D levels, with AA and AG genotypes having significantly lower serum 25 (OH) D levels as compared to GG [13.24 (8.4) ng/ml, 15.02 (7.07) ng/ml and 20.67 (13.64) ng/ml respectively]. There was no difference in HbA levels with respect to the vitamin D levels and VDR polymorphisms.
AA and AG genotypes of polymorphisms are associated with low serum 25 (OH) D levels. However there was no association between VDR polymorphisms and glycemic status in south Indian type 2 diabetes patients.
评估来自印度南部的2型糖尿病患者中多态性（、和）与维生素D水平及血糖状态之间的关联。
在这项观察性研究中，将200例2型糖尿病患者的基因型频率和维生素D水平与来自印度南部的300名对照（健康志愿者无关的匿名储存样本）进行比较。采用免疫分析技术测定血清25（OH）D水平，使用高效液相色谱法测定糖化血红蛋白（HbA），并使用实时聚合酶链反应（RT PCR）对维生素D受体（VDR）多态性进行基因分型。
发现约69.2%的2型糖尿病患者存在维生素D缺乏。多态性在血清25（OH）D水平上表现出差异，AA和AG基因型的血清25（OH）D水平显著低于GG型[分别为13.24（8.4）ng/ml、15.02（7.07）ng/ml和20.67（13.64）ng/ml]。关于维生素D水平和VDR多态性，HbA水平没有差异。
多态性的AA和AG基因型与低血清25（OH）D水平相关。然而，在印度南部2型糖尿病患者中，VDR多态性与血糖状态之间没有关联。
