Department of Hypertensiology, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, Poland.
Department of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, Poland.
J Mol Cell Cardiol. 2021 Jul;156:1-6. doi: 10.1016/j.yjmcc.2021.03.004. Epub 2021 Mar 14.
Various types of human endothelial cells, including human umbilical vein endothelial cells (HUVECs) and the established hybrid EAhy926 cells, are used in experimental research. Here, we compared the biological properties of HUVECs and EAhy926 cells under normal (5 mM) and high glucose (30 mM; HG) conditions. The results showed that HG induced cellular senescence and a stronger DNA damage response in HUVECs than in EAhy926 cells. The magnitude of oxidative stress elicited in HUVECs by HG was also greater than that elicited in their established counterparts. Both endothelial cell types promoted the progression of breast (MCF7), ovarian (OVCAR-3), and lung (A549) cancer cells; however, the effects elicited by HG-treated HUVECs on adhesion (MCF7, OVCAR-3), proliferation (OVCAR-3), and migration (OVCAR-3) were more pronounced. Finally, HG stimulated the production of a higher number of proangiogenic agents in HUVECs than in EAhy926 cells. Collectively, our study shows that the functional properties of primary and established endothelial cells exposed to HG differ substantially, which seems to result from the higher sensitivity of the former to this stressor. The interchangeability of both types of endothelial cells in biomedical research should be considered with great care to avoid losing some biological effects due to the choice of cells with higher stress tolerance.
各种类型的人内皮细胞，包括人脐静脉内皮细胞（HUVECs）和已建立的杂交 EAhy926 细胞，都被用于实验研究。在这里，我们比较了 HUVECs 和 EAhy926 细胞在正常（5mM）和高葡萄糖（30mM；HG）条件下的生物学特性。结果表明，HG 诱导 HUVECs 细胞衰老和更强的 DNA 损伤反应比 EAhy926 细胞。HG 引起的 HUVECs 氧化应激的幅度也大于其建立的对应物。这两种内皮细胞类型都促进了乳腺癌（MCF7）、卵巢癌（OVCAR-3）和肺癌（A549）癌细胞的进展；然而，HG 处理的 HUVECs 对粘附（MCF7、OVCAR-3）、增殖（OVCAR-3）和迁移（OVCAR-3）的影响更为明显。最后，HG 刺激 HUVECs 产生比 EAhy926 细胞更多的促血管生成剂。总之，我们的研究表明，暴露于 HG 的原代和已建立的内皮细胞的功能特性有很大差异，这似乎是由于前者对这种应激源更敏感。在生物医学研究中，应谨慎考虑两种类型的内皮细胞的可互换性，以避免由于选择具有更高耐受力的细胞而失去某些生物学效应。
