Baldwin K A, Forney R
Ventura County Sheriff's Criminalistics Laboratory, CA.
J Forensic Sci. 1988 Mar;33(2):462-9.
Succinylcholine (SCh) has been detected six months postmortem in liver, kidney, and injection site muscle of rats given 10 to 200 mg/kg by intramuscular injection. SCh stability was studied in canine tissue to evaluate three storage temperatures and two chemical preservatives at three time periods after injection. Nine mongrel dogs weighing 17.2 to 28 kg were divided equally into three groups and administered either 0.5, 1.0, or 5.0 mg SCh/kg intravenously into the cephalic vein. Liver, kidney, and gastrocnemius muscle were removed 90 min post-injection and divided into twelve portions. Each portion was treated with embalming fluid, physostigmine, the combination (50/50), or nothing. Chemically treated tissues and nontreated tissues were then stored at either 27, 5, or -20 degrees C for a period of up to forty days. Tissue portions were analyzed using ion-pair extraction, chemical demethylation, and gas chromatography with nitrogen phosphorous detection. Stability of SCh was greatest for samples stored at -20 degrees C and preserved with the combination of embalming fluid plus physostigmine. Kidney concentrations of SCh were significantly higher than those in liver or muscle at all doses. SCh was detected 24 h post-injection in all cases. By 40 days, only trace amounts of SCh, if any, could be detected in samples stored at room temperature with no chemical preservatives.
通过肌肉注射给大鼠注射10至200mg/kg琥珀酰胆碱（SCh）后，在大鼠的肝脏、肾脏和注射部位肌肉中，死后六个月仍可检测到SCh。在犬类组织中研究了SCh的稳定性，以评估注射后三个时间段的三种储存温度和两种化学防腐剂。将9只体重为17.2至28kg的杂种犬平均分为三组，通过头静脉静脉注射0.5、1.0或5.0mg SCh/kg。注射后90分钟取出肝脏、肾脏和腓肠肌并分成12份。每份分别用防腐液、毒扁豆碱、两者组合（50/50）处理，或不处理。然后将经化学处理的组织和未处理的组织储存在27、5或-20摄氏度下长达40天。使用离子对萃取、化学脱甲基和氮磷检测气相色谱法分析组织样本。对于储存在-20摄氏度并用防腐液加毒扁豆碱组合保存的样本，SCh的稳定性最高。在所有剂量下，肾脏中SCh的浓度均显著高于肝脏或肌肉中的浓度。在所有情况下，注射后24小时均可检测到SCh。到40天时，在没有化学防腐剂的室温储存样本中，只能检测到痕量的SCh（如果有的话）。
