Al-Ansari Mysoon M, Ranjit Singh A J A, Al-Khattaf Fatimah S, Michael J S
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Department of Biotechnology, Prathyusha Engineering College, Chennai 600056, India.
Saudi J Biol Sci. 2021 Mar;28(3):1596-1606. doi: 10.1016/j.sjbs.2020.12.005. Epub 2020 Dec 8.
Traditional medicine is becoming a primary source of health care in many countries in recent years. The current study proposes a new dimension of understanding a traditional origin treatment, using herbo-mineral preparations in nanoform. The herbo-mineral preparation [HMLC], was prepared according to the ancient palm script protocol dates back to 1000 years. In search of alternative therapy for the coronavirus, an attempt was made to determine this ethnic medicine formulation's therapeutic potential for viral hepatitis infection. The Hepatitis C virus [HCV] has several genomic similarities with SARS-CoV-2 viruses. The herbo-mineral formulation (HMLC) were analyzed using UV-vis, EDAX, FTIR, XRD, SEM, and TEM studies. SEM images confirmed the ' presence of nanoparticles with agglomerated conditions having an average grain size of 18 to 25 nm. EDAX studies showed the presence of metallic components in oxide or sulfide form in HMLC. The HCV inhibitory effects of HMLC indicated a good response. The cytotoxicity of this preparation against the Huh-7 human hepatoma cell line was significant. The HMLC showed a strong inhibitory effect on HCV replication in a dose-dependent manner. The genomic component of HCV is similar to COVID -19 virus. The Hepatitis C virus (HCV) NS3/4A protease has a striking three-dimensional structural similarity to the SARS-CoV2 M protease, particularly in the arrangement of key active site residues. So HMLC can be tried to treat coronavirus infection. At higher concentrations, HMLC exhibited over 100-fold inhibition. In the MTT assay, HMLC did not show any apparent cytotoxic effect on cell viability at the concentrations 1-100 µg. Histological studies indicated that the liver and kidney did not experience any toxicity by 7 and 15 consecutive days of administration of HMLC on experimental Wistar rats. Hence, the HMLC can be tried as a therapy for COVID -19 infections using the preparations strictly according to ethnopharmacological protocol and optimum doses.
近年来，传统医学正在成为许多国家医疗保健的主要来源。当前的研究提出了一个理解传统起源治疗的新维度，即使用纳米形式的草药-矿物制剂。这种草药-矿物制剂 [HMLC] 是根据可追溯到1000年前的古代棕榈叶手稿协议制备的。为了寻找针对冠状病毒的替代疗法，人们尝试确定这种民族药物制剂对病毒性肝炎感染的治疗潜力。丙型肝炎病毒 [HCV] 与SARS-CoV-2病毒有几个基因组相似之处。使用紫外-可见光谱、能谱分析、傅里叶变换红外光谱、X射线衍射、扫描电子显微镜和透射电子显微镜研究对草药-矿物制剂（HMLC）进行了分析。扫描电子显微镜图像证实存在团聚状态的纳米颗粒，平均粒径为18至25纳米。能谱分析研究表明HMLC中存在氧化物或硫化物形式的金属成分。HMLC对HCV的抑制作用显示出良好的反应。该制剂对Huh-7人肝癌细胞系的细胞毒性显著。HMLC对HCV复制呈剂量依赖性的强烈抑制作用。HCV的基因组成分与新冠病毒相似。丙型肝炎病毒（HCV）的NS3/4A蛋白酶与SARS-CoV2的M蛋白酶在三维结构上有显著相似性，特别是在关键活性位点残基的排列上。因此，可以尝试使用HMLC治疗冠状病毒感染。在较高浓度下，HMLC表现出超过100倍的抑制作用。在MTT试验中，HMLC在1-100微克/毫升的浓度下对细胞活力未显示任何明显的细胞毒性作用。组织学研究表明，在实验性Wistar大鼠连续7天和15天给予HMLC后，肝脏和肾脏未出现任何毒性。因此，可以按照民族药理学协议和最佳剂量严格使用该制剂，尝试将HMLC作为新冠病毒感染的一种治疗方法。
