Alsalhi Mohamad S, Devanesan Sandhanasamy, Alfuraydi Akram A, Vishnubalaji Radhakrishnan, Munusamy Murugan A, Murugan Kadarkarai, Nicoletti Marcello, Benelli Giovanni
Research Chair in Laser Diagnosis of Cancers; Department of Physics and Astronomy.
Department of Botany and Microbiology, College of Science.
Int J Nanomedicine. 2016 Sep 6;11:4439-4449. doi: 10.2147/IJN.S113193. eCollection 2016.
The present study focused on a simple and eco-friendly method for the synthesis of silver nanoparticles (AgNPs) with multipurpose anticancer and antimicrobial activities.
We studied a green synthesis route to produce AgNPs by using an aqueous extract of seeds (3 mM). Their antimicrobial activity and cytotoxicity on human neonatal skin stromal cells (hSSCs) and colon cancer cells (HT115) were assessed.
A biophysical characterization of the synthesized AgNPs was realized: the morphology of AgNPs was determined by transmission electron microscopy, energy dispersive spectroscopy, X-ray powder diffraction, and ultraviolet-vis absorption spectroscopy. Transmission electron microscopy showed spherical shapes of AgNPs of seed extracts with a 3.2 nm minimum diameter and average diameter ranging from 3.2 to 16 nm. X-ray powder diffraction highlighted the crystalline nature of the nanoparticles, ultraviolet-vis absorption spectroscopy was used to monitor their synthesis, and Fourier transform infrared spectroscopy showed the main reducing groups from the seed extract. Energy dispersive spectroscopy was used to confirm the presence of elemental silver. We evaluated the antimicrobial potential of green-synthesized AgNPs against five infectious bacteria: (29213), (4436), (G455), , and In addition, we focused on the toxicological effects of AgNPs against hSSC cells and HT115 cells by using in vitro proliferation tests and cell viability assays. Among the different tested concentrations of nanoparticles, doses < 10 µg showed few adverse effects on cell proliferation without variations in viability, whereas doses >10 µg led to increased cytotoxicity.
Overall, our results highlighted the capacity of -synthesized AgNPs as novel and cheap bioreducing agents for eco-friendly nanosynthetical routes. The data confirm the multipurpose potential of plant-borne reducing and stabilizing agents in nanotechnology.
本研究聚焦于一种简单且环保的合成具有多种抗癌和抗菌活性的银纳米颗粒（AgNPs）的方法。
我们研究了一种绿色合成路线，通过使用种子水提取物（3 mM）来制备AgNPs。评估了它们对人新生儿皮肤基质细胞（hSSCs）和结肠癌细胞（HT115）的抗菌活性和细胞毒性。
对合成的AgNPs进行了生物物理表征：通过透射电子显微镜、能量色散光谱、X射线粉末衍射和紫外可见吸收光谱确定了AgNPs的形态。透射电子显微镜显示种子提取物的AgNPs呈球形，最小直径为3.2 nm，平均直径在3.2至16 nm之间。X射线粉末衍射突出了纳米颗粒的晶体性质，紫外可见吸收光谱用于监测它们的合成，傅里叶变换红外光谱显示了种子提取物中的主要还原基团。能量色散光谱用于确认元素银的存在。我们评估了绿色合成的AgNPs对五种感染性细菌的抗菌潜力：（29213）、（4436）、（G455）、 和 此外，我们通过体外增殖试验和细胞活力测定，重点研究了AgNPs对hSSC细胞和HT115细胞的毒理学效应。在不同测试浓度的纳米颗粒中，剂量<10 µg对细胞增殖几乎没有不良影响，活力无变化，而剂量>10 µg导致细胞毒性增加。
总体而言，我们的结果突出了合成的AgNPs作为新型且廉价的生物还原剂用于环保纳米合成路线的能力。数据证实了植物源性还原剂和稳定剂在纳米技术中的多种用途潜力。
