Pachla Artur, Ptaszyńska Aneta A, Wicha Magdalena, Kunat Magdalena, Wydrych Jerzy, Oleńska Ewa, Małek Wanda
Research and Development Center, Biowet Puławy, 2 H. Arciucha st., 24-100 Puławy, Poland.
Department of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, 19 Akademicka st., 20-033 Lublin, Poland.
Saudi J Biol Sci. 2021 Mar;28(3):1890-1899. doi: 10.1016/j.sjbs.2020.12.040. Epub 2021 Jan 1.
Taking into account that fructophilic lactic acid bacteria (FLAB) can play an important role in the health of honey bees and can be used as probiotics, phenotypic properties of probiotic interest of (12 strains) and bacteria (2 strains), isolated from gastrointestinal tract, have been studied. We have evaluated survival of tested FLAB in honey bee gut, their susceptibility to antibiotics (ampicillin, erythromycin, tylosin), cell surface hydrophobicity, auto-aggregation ability, co-aggregation with model pathogenic bacteria, biofilm formation capacity, and effect of studied FLAB, added to sucrose syrup bee diet, on longevity of honey bees. The tested FLAB exhibited good gastrointestinal tract tolerance and high antibiotic susceptibility, which are important criteria in the screening of probiotic candidates. It was also found that all FLAB studied have high cell surface hydrophobicity and fulfil next selection criterion for their use as probiotics. Symbionts of showed also auto- and co-aggregation capacities regarded as valuable features for biofilm formation and inhibition of pathogens adhesion to the bee gut cells. Biofilm-development ability is a desired characteristic of probiotic lactic acid bacteria. As indicated by quantitative crystal violet-stained microplate assay and confocal laser scanning microscopy imaging, all studied gut isolates exhibit a biofilm positive phenotype. Moreover, it was also documented, on honey bees kept in cages, that supplementation of sucrose diet with FLAB decreases mortality and improves significantly longevity of honey bees. Presented research showed that FLAB symbionts are good candidates for application as probiotics.
考虑到嗜果糖乳酸菌（FLAB）对蜜蜂健康具有重要作用且可作为益生菌使用，我们对从蜜蜂胃肠道分离出的12株嗜果糖乳酸菌和2株细菌的益生菌相关表型特性进行了研究。我们评估了受试嗜果糖乳酸菌在蜜蜂肠道中的存活率、它们对抗生素（氨苄青霉素、红霉素、泰乐菌素）的敏感性、细胞表面疏水性、自聚集能力、与模式病原菌的共聚集能力、生物膜形成能力，以及添加到蔗糖糖浆蜜蜂饲料中的受试嗜果糖乳酸菌对蜜蜂寿命的影响。受试嗜果糖乳酸菌表现出良好的胃肠道耐受性和较高的抗生素敏感性，这是筛选益生菌候选菌株的重要标准。还发现所有研究的嗜果糖乳酸菌都具有较高的细胞表面疏水性，满足其作为益生菌使用的下一个选择标准。蜜蜂的共生菌还表现出自聚集和共聚集能力，这被认为是生物膜形成以及抑制病原菌黏附于蜜蜂肠道细胞的重要特性。生物膜形成能力是益生菌乳酸菌的一个理想特性。通过定量结晶紫染色微孔板测定和共聚焦激光扫描显微镜成像表明，所有研究的蜜蜂肠道分离株均表现出生物膜阳性表型。此外，在笼养蜜蜂中也有记录表明，在蔗糖饲料中添加嗜果糖乳酸菌可降低死亡率并显著提高蜜蜂的寿命。目前的研究表明，嗜果糖乳酸菌共生菌是作为益生菌应用的良好候选菌株。
