Hashim Ahmed M, Alatawi Aishah, Altaf Faris M, Qari Sameer H, Elhady Mohamed E, Osman Gamal H, Abouseadaa Heba H
Botany Department, Faculty of Science, Ain Shams University, Cairo 11865, Egypt.
Biology Department, Faculty of Science, Tabuk University, Tabuk 71421, Saudi Arabia.
Saudi J Biol Sci. 2021 Mar;28(3):1919-1930. doi: 10.1016/j.sjbs.2020.12.043. Epub 2021 Jan 1.
A high degree of endemism has been recorded for several plant groups collectively in Saint Katherine Protectorate (SKP) in the Sinai Peninsula. Nine endangered endemic plant species in SKP were selected to test the variable abilities of three different DNA barcodes; Riboluse-1,5- Biphosphate Carboxylase/Oxygenase Large subunit (), Internal Transcribed Spacer (), and the two regions of the plastid gene () as well as Start Codon Targeted (SCoT) Polymorphism to find the phylogenetic relationships among them. The three barcodes were generally more capable of finding the genetic relationships among the plant species under study, new barcodes were introduced to the National Centre for Biotechnology Information (NCBI) for the first time through our work. The barcode sequences were efficient in finding the genetic relationships between the nine species. However, SCoT polymorphism could only cluster plant species belonging to the same genus together in one group, but it could not cluster plant species belonging to the same families except for some primers solely. was the most easily amplified and identified barcode in eight out of the nine species at the species level and the ninth barcode to the genus level. identified all the species to the genus level. Finally, identified six out of the eight species, but it could not identify two of the eight species to the genus level.
在西奈半岛的圣凯瑟琳保护区（SKP），已记录到几种植物类群具有高度的特有性。从SKP中选择了9种濒危特有植物物种，以测试三种不同DNA条形码的可变能力；核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基（）、内转录间隔区（）、质体基因的两个区域（）以及起始密码子靶向（SCoT）多态性，以确定它们之间的系统发育关系。这三种条形码通常更有能力找出所研究植物物种之间的遗传关系，通过我们的工作，新的条形码首次被引入到美国国立生物技术信息中心（NCBI）。条形码序列在找出这9个物种之间的遗传关系方面很有效。然而，SCoT多态性只能将属于同一属的植物物种聚集在一组中，但除了一些引物外，它不能将属于同一科的植物物种聚集在一起。在9个物种中的8个物种的物种水平以及第9个物种的属水平上，是最容易扩增和鉴定的条形码。将所有物种鉴定到属水平。最后，鉴定出了8个物种中的6个，但它不能将8个物种中的2个鉴定到属水平。
