Abouseadaa Heba H, Atia Mohamed A M, Younis Inas Y, Issa Marwa Y, Ashour Haraz A, Saleh Ibrahim, Osman Gamal H, Arif Ibrahim A, Mohsen Engy
Botany Department, Faculty of Science, Ain-shams University, Egypt.
Molecular Genetics and Genome Mapping Lab., Agriculture Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Egypt.
Saudi J Biol Sci. 2020 Jun;27(6):1649-1658. doi: 10.1016/j.sjbs.2020.03.007. Epub 2020 Mar 12.
Cactaceae plant family comprises over 130 genera and 2000 species of succulent flowering plants. The genera and (), which have medicinal and nutritional applications as well as aesthetic appeal, are considered to be among the major genera of the family. Several species of both genera show morphological and chemical similarities and diversities according to environmental conditions and genotypes. Here, we assessed the genetic relationships of nine species belonging to two major genera and under the family Cactaceae, using two modern gene-targeting marker techniques, the Start Codon Targeted (SCoT) Polymorphism and the Conserved DNA-Derived Polymorphism (CDDP). Besides, we screened the various phytochemicals and evaluated the antioxidant activities of the nine species of cacti. Five out of the 10 SCoT and eight CDDP primers used to screen genetic variations within the nine species yielded species-specific reproducible bands. The entire 156 loci were detected, of which 107 were polymorphic, 26 were monomorphic, and 23 were unique loci. The nine species were categorized into two groups based on the dendrogram and similarity matrix. Phytochemical profiling revealed that sterols, triterpenes, flavonoids, and tannins were found in all the tested species. Additionally, two species ( and ) and one species () revealed a considerable antioxidant activity. Our results demonstrated that gene-targeting marker techniques were highly powerful tools for the classification and characterization of the nine investigated species, despite displaying high similarities at both morphological and phytochemical levels.
仙人掌科植物家族包含130多个属和2000种多肉开花植物。仙人球属和量天尺属（）具有药用、营养用途以及观赏价值，被认为是该科的主要属。这两个属的几个物种根据环境条件和基因型表现出形态和化学上的相似性和多样性。在这里，我们使用两种现代基因靶向标记技术，即起始密码子靶向（SCoT）多态性和保守DNA衍生多态性（CDDP），评估了仙人掌科两个主要属仙人球属和量天尺属的九个物种之间的遗传关系。此外，我们筛选了各种植物化学物质，并评估了这九种仙人掌的抗氧化活性。用于筛选这九个物种内遗传变异的10个SCoT引物和8个CDDP引物中有5个产生了物种特异性的可重复条带。共检测到156个位点，其中107个是多态性的，26个是单态性的，23个是独特位点。根据树状图和相似性矩阵，这九个物种被分为两组。植物化学分析表明，所有测试物种中都发现了甾醇、三萜、黄酮类化合物和单宁。此外，两种仙人球属植物（和）和一种量天尺属植物（）表现出相当高的抗氧化活性。我们的结果表明，尽管这九个被研究物种在形态和植物化学水平上表现出高度相似性，但基因靶向标记技术是对它们进行分类和表征的强大工具。
