Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
Division of Molecular Biology and Human Genetics, Department of Science and Technology-National Research Foundation (DST-NRF) Centre of Excellence for Biomedical Tuberculosis Research, Faculty of Medicine and Health Sciences, South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.
Front Immunol. 2021 Feb 24;12:608846. doi: 10.3389/fimmu.2021.608846. eCollection 2021.
Several host inflammatory markers have been proposed as biomarkers for diagnosis and treatment response in Tuberculosis (TB), but few studies compare their utility in different demographic, ethnic, and TB endemic settings. Fifty-four host biomarkers were evaluated in plasma samples obtained from presumed TB cases recruited at the Oslo University Hospital in Norway, and a health center in Cape Town, South Africa. Based on clinical and laboratory assessments, participants were classified as having TB or other respiratory diseases (ORD). The concentrations of biomarkers were analyzed using the Luminex multiplex platform. Out of 185 study participants from both study sites, 107 (58%) had TB, and 78 (42%) ORD. Multiple host markers showed diagnostic potential in both the Norwegian and South African cohorts, with I-309 as the most accurate single marker irrespective of geographical setting. Although study site-specific biosignatures had high accuracy for TB, a site-independent 5-marker biosignature (G-CSF, C3b/iC3b, procalcitonin, IP-10, PDGF-BB) was identified diagnosing TB with a sensitivity of 72.7% (95% CI, 49.8-82.3) and specificity of 90.5% (95% CI, 69.6-98.8) irrespective of geographical site. A 5-marker host plasma biosignature has diagnostic potential for TB disease irrespective of TB setting and should be further explored in larger cohorts.
几种宿主炎症标志物已被提出作为结核病（TB）诊断和治疗反应的生物标志物，但很少有研究比较它们在不同的人口统计学、种族和结核病流行地区的实用性。评估了来自挪威奥斯陆大学医院和南非开普敦的一个保健中心的疑似结核病患者的血浆样本中的 54 种宿主生物标志物。根据临床和实验室评估，将参与者分为结核病或其他呼吸道疾病（ORD）。使用 Luminex 多重平台分析了生物标志物的浓度。来自两个研究地点的 185 名研究参与者中，107 人（58%）患有结核病，78 人（42%）患有 ORD。多个宿主标志物在挪威和南非队列中均显示出诊断潜力，I-309 是最准确的单一标志物，与地理位置无关。尽管特定于研究地点的生物标志物对结核病具有高准确性，但确定了一个与地理位置无关的 5 标志物生物标志物（G-CSF、C3b/iC3b、降钙素原、IP-10、PDGF-BB），其诊断结核病的敏感性为 72.7%（95%CI，49.8-82.3），特异性为 90.5%（95%CI，69.6-98.8），与地理位置无关。一种 5 标志物宿主血浆生物标志物具有诊断结核病疾病的潜力，与结核病环境无关，应在更大的队列中进一步探索。
