Rakocević S, Silobrcić V
Institute of Immunology, Zagreb, Yugoslavia.
J Biol Response Mod. 1988 Feb;7(1):6-10.
Peptidoglycan monomer (dissacharide-pentapeptide, PGM) from Brevibacterium divaricatum is an immunomodulator and an antitumor agent. As part of our investigation of the antitumor properties of PGM in mice, its potential to stimulate phagocytosis by resident peritoneal macrophages was assessed. Inbred CBA/H/Zg tau mice (kept under conventional conditions) were used, and a simple and brief method was developed to evaluate phagocytosis. It consisted of a short (10 min) coincubation of yeast cells (YCs) with peritoneal cells (PCs) and microscopic (phase-contrast) scoring of YC ingestion. Three samples of PGM were injected intravenously into mice (60 mg/kg), and PCs were collected from groups of recipients 8, 16, 24, 48, or 72 h later. All samples temporarily increased phagocytosis, but the extent and duration of the effect varied. Stimulation of phagocytosis also occurred after in vitro exposure (3 h) of PCs to PGM, or to the pentapeptide (PP) part of its molecule. We concluded that PGM could enhance phagocytosis by resident peritoneal macrophages in vivo and in vitro, the PP being the active component in vitro.
来自分歧短杆菌的肽聚糖单体（双糖 - 五肽，PGM）是一种免疫调节剂和抗肿瘤剂。作为我们对PGM在小鼠体内抗肿瘤特性研究的一部分，评估了其刺激驻留腹膜巨噬细胞吞噬作用的潜力。使用近交系CBA/H/Zg tau小鼠（饲养在常规条件下），并开发了一种简单且简短的方法来评估吞噬作用。该方法包括酵母细胞（YCs）与腹膜细胞（PCs）短时间（10分钟）共孵育，以及对YC摄取进行显微镜（相差）评分。将三份PGM样品静脉注射到小鼠体内（60毫克/千克），并在8、16、24、48或72小时后从受体组中收集PCs。所有样品均使吞噬作用暂时增加，但作用的程度和持续时间有所不同。在PCs体外暴露于PGM或其分子的五肽（PP）部分3小时后，也会发生吞噬作用的刺激。我们得出结论，PGM可以在体内和体外增强驻留腹膜巨噬细胞的吞噬作用，PP是体外的活性成分。
