Gund Rupali, Zirmire Ravindra, J Haarshaadri, Kansagara Gaurav, Jamora Colin
Department of Dermatology, Columbia University, New York, USA.
IFOM-inStem Joint Research Laboratory, Centre for Inflammation and Tissue Homeostasis, Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, Karnataka, India.
Bio Protoc. 2021 Jan 20;11(2):e3894. doi: 10.21769/BioProtoc.3894.
The skin is the largest organ that protects our body from the external environment and it is constantly exposed to pathogenic insults and injury. Repair of damage to this organ is carried out by a complex process involving three overlapping phases of inflammation, proliferation and remodeling. Histological analysis of wounded skin is a convenient approach to examine broad alterations in tissue architecture and investigate cells in their indigenous microenvironment. In this article we present a protocol for immunohistochemical examination of wounded skin to study mechanisms involved in regulating stem cell activity, which is a vital component in the repair of the damaged tissue. Performing such histological analysis enables the understanding of the spatial relationship between cells that interact in the specialized wound microenvironment. The analytical tools described herein permit the quantitative measurement of the regenerative ability of stem cells adjacent to the wound and the extent of re-epithelialization during wound closure. These protocols can be adapted to investigate numerous cellular processes and cell types within the wounded skin.
皮肤是保护我们身体免受外部环境侵害的最大器官，它不断受到病原体侵袭和损伤。该器官损伤的修复是一个复杂的过程，涉及炎症、增殖和重塑三个重叠阶段。对受伤皮肤进行组织学分析是检查组织结构广泛变化并在其原生微环境中研究细胞的便捷方法。在本文中，我们提出了一种用于受伤皮肤免疫组织化学检查的方案，以研究参与调节干细胞活性的机制，干细胞活性是受损组织修复的重要组成部分。进行这种组织学分析能够了解在特殊伤口微环境中相互作用的细胞之间的空间关系。本文所述的分析工具可对伤口附近干细胞的再生能力以及伤口闭合过程中再上皮化的程度进行定量测量。这些方案可用于研究受伤皮肤内的众多细胞过程和细胞类型。
