果蝇三龄幼虫神经肌肉接头的免疫电生理学研究
Immuno-electrophysiology on Neuromuscular Junctions of Drosophila Third Instar Larva.
作者信息
Klima Raffaella, Romano Giulia, Gbadamosi Monsurat, Megighian Aram, Feiguin Fabian
机构信息
International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149 Trieste, Italy.
Department of Biomedical Sciences, University of Padova, via Marzolo 3, 35131 Padova, Italy.
出版信息
Bio Protoc. 2021 Feb 5;11(3):e3913. doi: 10.21769/BioProtoc.3913.
Abstract
Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through immune-electrophysiology in . This technique allows the quantification of the molecular behavior of the neuromuscular synapses by correlating the structural configuration of the synaptic boutons with their electrical activity.
摘要
突触传递的改变是神经肌肉疾病早期的关键事件。然而，仍需要可靠的方法来分析神经肌肉突触的功能组织。本手稿提供了一种详细的方案，用于通过免疫电生理学分析神经肌肉突触的分子组装。该技术通过将突触小体的结构配置与其电活动相关联，实现对神经肌肉突触分子行为的量化。
相似文献
1
Immuno-electrophysiology on Neuromuscular Junctions of Drosophila Third Instar Larva.果蝇三龄幼虫神经肌肉接头的免疫电生理学研究
Bio Protoc. 2021 Feb 5;11(3):e3913. doi: 10.21769/BioProtoc.3913.
2
Modulation of neuromuscular synapses and contraction in Drosophila 3rd instar larvae.果蝇三龄幼虫神经肌肉突触与收缩的调节
J Neurogenet. 2018 Sep;32(3):183-194. doi: 10.1080/01677063.2018.1502761. Epub 2018 Oct 10.
3
Focal recording of synaptic currents from single boutons at the Drosophila neuromuscular junction.在果蝇神经肌肉接头处对单个突触小体的突触电流进行局部记录。
Cold Spring Harb Protoc. 2010 Sep 1;2010(9):pdb.prot5489. doi: 10.1101/pdb.prot5489.
4
Morphological and functional effects of altered cysteine string protein at the Drosophila larval neuromuscular junction.果蝇幼虫神经肌肉接头处半胱氨酸串珠蛋白改变的形态学和功能效应。
Synapse. 2007 Jan;61(1):1-16. doi: 10.1002/syn.20335.
5
Analysis of synaptic growth and function in Drosophila with an extended larval stage.延长果蝇幼虫期以分析突触生长和功能。
J Neurosci. 2012 Oct 3;32(40):13776-86. doi: 10.1523/JNEUROSCI.0508-12.2012.
6
Long-term in vitro maintenance of neuromuscular junction activity of Drosophila larvae.果蝇幼虫神经肌肉接头活性的长期体外维持
Comp Biochem Physiol A Mol Integr Physiol. 2003 Feb;134(2):247-55. doi: 10.1016/s1095-6433(02)00243-x.
7
In vivo imaging of Drosophila larval neuromuscular junctions to study synapse assembly.利用果蝇幼虫神经肌肉接头的体内成像研究突触组装。
Cold Spring Harb Protoc. 2012 Apr 1;2012(4):407-13. doi: 10.1101/pdb.top068577.
8
Chronic lead exposure alters presynaptic calcium regulation and synaptic facilitation in Drosophila larvae.慢性铅暴露会改变果蝇幼虫突触前钙调节和突触易化。
Neurotoxicology. 2009 Sep;30(5):777-84. doi: 10.1016/j.neuro.2009.08.007. Epub 2009 Sep 2.
9
Developmental consequences of neuromuscular junctions with reduced presynaptic calcium channel function.突触前钙通道功能降低的神经肌肉接头的发育后果。
Synapse. 2005 Sep 1;57(3):132-47. doi: 10.1002/syn.20165.
10
Miniature endplate currents at the newly formed neuromuscular junction in Drosophila embryos and larvae.果蝇胚胎和幼虫中新形成的神经肌肉接头处的微小终板电流。
Neurosci Res. 1994 Mar;19(2):143-54. doi: 10.1016/0168-0102(94)90137-6.
本文引用的文献
1
TDP-43 promotes the formation of neuromuscular synapses through the regulation of Disc-large expression in Drosophila skeletal muscles.TDP-43 通过调节果蝇骨骼肌中的 Disc-large 表达促进神经肌肉突触的形成。
BMC Biol. 2020 Mar 26;18(1):34. doi: 10.1186/s12915-020-00767-7.
2
Estimation of the Readily Releasable Synaptic Vesicle Pool at the Larval Neuromuscular Junction.幼虫神经肌肉接头处易释放突触小泡池的估计
Bio Protoc. 2019 Jan 5;9(1). doi: 10.21769/BioProtoc.3127.
3
FM1-43 Photoconversion and Electron Microscopy Analysis at the Neuromuscular Junction.神经肌肉接头处的FM1-43光转化与电子显微镜分析
Bio Protoc. 2017 Sep 5;7(17). doi: 10.21769/BioProtoc.2523.
4
Glial TDP-43 regulates axon wrapping, GluRIIA clustering and fly motility by autonomous and non-autonomous mechanisms.神经胶质细胞中的TDP-43通过自主和非自主机制调节轴突包裹、GluRIIA聚集和果蝇运动能力。
Hum Mol Genet. 2015 Nov 1;24(21):6134-45. doi: 10.1093/hmg/ddv330. Epub 2015 Aug 13.
5
Chronological requirements of TDP-43 function in synaptic organization and locomotive control.TDP-43功能在突触组织和运动控制中的时间顺序要求。
Neurobiol Dis. 2014 Nov;71:95-109. doi: 10.1016/j.nbd.2014.07.007. Epub 2014 Aug 1.
6
TDP-43 regulates Drosophila neuromuscular junctions growth by modulating Futsch/MAP1B levels and synaptic microtubules organization.TDP-43 通过调节 Futsch/MAP1B 水平和突触微管组织来调控果蝇神经肌肉接头的生长。
PLoS One. 2011 Mar 11;6(3):e17808. doi: 10.1371/journal.pone.0017808.
7
Arg206 of SNAP-25 is essential for neuroexocytosis at the Drosophila melanogaster neuromuscular junction.SNAP-25 的 Arg206 对于果蝇肌神经接点的神经递质释放是必需的。
J Cell Sci. 2010 Oct 1;123(Pt 19):3276-83. doi: 10.1242/jcs.071316. Epub 2010 Sep 7.
8
Depletion of TDP-43 affects Drosophila motoneurons terminal synapsis and locomotive behavior.TDP-43 的缺失会影响果蝇运动神经元的终末突触和运动行为。
FEBS Lett. 2009 May 19;583(10):1586-92. doi: 10.1016/j.febslet.2009.04.019. Epub 2009 Apr 19.
9
The drosophila neuromuscular junction: a model system for studying synaptic development and function.果蝇神经肌肉接头：用于研究突触发育和功能的模型系统。
Annu Rev Neurosci. 1996;19:545-75. doi: 10.1146/annurev.ne.19.030196.002553.
10
Improved stability of Drosophila larval neuromuscular preparations in haemolymph-like physiological solutions.果蝇幼虫神经肌肉制剂在类血淋巴生理溶液中的稳定性提高。
J Comp Physiol A. 1994 Aug;175(2):179-91. doi: 10.1007/BF00215114.
Zotero 插件