Klima Raffaella, Romano Giulia, Gbadamosi Monsurat, Megighian Aram, Feiguin Fabian
International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149 Trieste, Italy.
Department of Biomedical Sciences, University of Padova, via Marzolo 3, 35131 Padova, Italy.
Bio Protoc. 2021 Feb 5;11(3):e3913. doi: 10.21769/BioProtoc.3913.
Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through immune-electrophysiology in . This technique allows the quantification of the molecular behavior of the neuromuscular synapses by correlating the structural configuration of the synaptic boutons with their electrical activity.
突触传递的改变是神经肌肉疾病早期的关键事件。然而，仍需要可靠的方法来分析神经肌肉突触的功能组织。本手稿提供了一种详细的方案，用于通过免疫电生理学分析神经肌肉突触的分子组装。该技术通过将突触小体的结构配置与其电活动相关联，实现对神经肌肉突触分子行为的量化。
