Oteiza P I, Hurley L S, Lönnerdal B, Keen C L
Department of Nutrition, University of California, Davis 95616.
J Nutr. 1988 Jun;118(6):735-8. doi: 10.1093/jn/118.6.735.
The possible physiological role of Zn in tubulin polymerization was studied. Tubulin assembly in vitro was characterized in brain supernatants from rats fed a marginally Zn-deficient diet (10 micrograms Zn/g) during pregnancy and lactation. The initial rate of tubulin polymerization was significantly lower (27%) in brain supernatants from the marginally Zn-deficient animals than from controls. This was associated with a lower Zn concentration in the brain supernatants from the marginally deficient rats. Total protein and tubulin concentrations were the same in the supernatants from both groups. These data show that Zn is necessary for normal tubulin assembly and indicate that one biochemical defect underlying brain alterations in Zn deficiency could be abnormal microtubule function.
研究了锌在微管蛋白聚合中可能的生理作用。对在妊娠和哺乳期喂食轻度缺锌饮食（10微克锌/克）的大鼠脑匀浆上清液中的微管蛋白组装进行了表征。轻度缺锌动物脑匀浆上清液中微管蛋白聚合的初始速率明显低于对照组（27%）。这与轻度缺锌大鼠脑匀浆上清液中较低的锌浓度有关。两组上清液中的总蛋白和微管蛋白浓度相同。这些数据表明，锌是正常微管蛋白组装所必需的，并表明锌缺乏时脑改变的一个潜在生化缺陷可能是微管功能异常。
