Banihani Saleem A, Khaled Hebah J
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.
Andrologia. 2021 Jul;53(6):e14052. doi: 10.1111/and.14052. Epub 2021 Mar 17.
Even though the effect of caffeine on humans' health has been revealed in various research studies, its effect on semen quality has yet to be well explained. Here, we measured the effect of caffeine at 1, 5, 10 and 20 mM on motility of human spermatozoa in normozoospermic and asthenozoospermic semen samples, level of seminal nitric oxide, chelation of seminal calcium ions and activity of seminal creatine kinase. Fifty-one normozoospermic and ten asthenozoospermic semen samples were recruited in this study. Sperm motility was evaluated by Makler counter, and seminal nitric oxide, seminal-free calcium and activity of seminal creatine kinase were measured spectrophotometrically. Caffeine at 10 mM significantly (p < .05) increased progressive motility of spermatozoa in both tested groups. Also, caffeine significantly increased (p < .05) activity of creatine kinase and insignificantly (p > .05) altered nitric oxide and free calcium levels in seminal plasma. In conclusion, progressive motility of human spermatozoa was found to be higher in the presence of caffeine at 10 mM in normozoospermic and asthenozoospermic semen samples; this increase, albeit partially, could be due to increased activity of seminal creatine kinase, but not to increased production of nitric oxide or chelation of free calcium ions.
尽管咖啡因对人类健康的影响已在各种研究中得到揭示，但其对精液质量的影响尚未得到充分解释。在此，我们测定了1、5、10和20毫摩尔咖啡因对正常精子症和弱精子症精液样本中人类精子活力、精液一氧化氮水平、精液钙离子螯合作用以及精液肌酸激酶活性的影响。本研究招募了51份正常精子症和10份弱精子症精液样本。通过Makler计数器评估精子活力，并用分光光度法测定精液一氧化氮、游离精液钙和精液肌酸激酶活性。10毫摩尔的咖啡因显著（p < 0.05）提高了两个测试组中精子的前向运动能力。此外，咖啡因显著提高（p < 0.05）了肌酸激酶活性，而对精液中一氧化氮和游离钙水平的改变不显著（p > 0.05）。总之，发现在正常精子症和弱精子症精液样本中，10毫摩尔咖啡因存在时人类精子的前向运动能力更高；这种增加，尽管只是部分原因，可能是由于精液肌酸激酶活性增加，而不是由于一氧化氮产生增加或游离钙离子螯合作用增强。
