Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo, P. R. China.
Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology, Beijing, P. R. China.
J Sep Sci. 2021 May;44(10):2143-2152. doi: 10.1002/jssc.202001216. Epub 2021 Apr 9.
In this work, a magnetic graphene material coated with mesoporous silica was selected as the substrate, 3-glycidoxypropyltrimethoxysilane and polyethyleneimine were sequentially bonded through chemical reactions, and then carrageenan was successfully introduced by electrostatic interaction; finally, hydrophilic nanocomposite material was prepared. Due to the large number of hydrophilic groups, and polyethyleneimine was connected by means of chemical bonds, this material exhibits good hydrophilicity and stability for glycopeptide enrichment. In the actual enrichment process, nanomaterial exhibits high selectivity (1:500), high sensitivity (2 fmol), and good repeatability (five cycles). In addition, the synthesized material also shows a good enrichment effect in the face of actual complex biological samples, which captured 40 N-glycopeptides from human saliva, indicating the application potential for enrichment of N-glycopeptides.
在这项工作中，选择了一种包覆有介孔硅的磁性石墨烯材料作为基底，通过化学反应依次键合 3-缩水甘油丙基三甲氧基硅烷和聚乙烯亚胺，然后通过静电相互作用成功引入卡拉胶；最终制备出亲水性纳米复合材料。由于具有大量的亲水基团，并且通过化学键合连接聚乙烯亚胺，该材料在糖肽富集方面表现出良好的亲水性和稳定性。在实际的富集过程中，纳米材料表现出高选择性（1:500）、高灵敏度（2 fmol）和良好的重复性（5 个循环）。此外，该合成材料在面对实际复杂的生物样品时也表现出良好的富集效果，从人唾液中捕获了 40 个 N-糖肽，表明其在 N-糖肽富集方面具有应用潜力。
