Department of Biochemistry,College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Sultanate of Oman.
Department of Medicine, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Sultanate of Oman.
Genet Test Mol Biomarkers. 2021 Mar;25(3):179-186. doi: 10.1089/gtmb.2020.0166.
is a transcription factor that is overexpressed in the early stages of ovarian cancer and has been suggested as a potential biomarker for early detection. In this study, we aimed to examine the role of in invasion and proliferation of ovarian cancer cells. We performed cell viability, colony formation, and invasion assays using ovarian cancer cells treated with siRNA to knock down the gene. The regulatory effects of on proteins involved in the apoptotic, Wnt, Hippo, and retinoblastoma signaling pathways were evaluated by western blotting following repression. In addition, we analyzed data available on Gene Expression Profiling Interactive Analysis for correlations between and , β, , , and . was highly expressed in ovarian cancer cell lines and samples when compared to the nonmalignant tissues. Downregulation of inhibited cell viability and invasion and promoted the phosphorylation of YAP, GSK-3-β, β-catenin, and retinoblastoma. However, cyclin D1, cdk4, and caspase-9 were downregulated when compared to control. Overall, promotes ovarian carcinogenesis via the regulation of Hippo and Wnt pathways
是一种转录因子，在卵巢癌的早期过度表达，并被认为是早期检测的潜在生物标志物。在这项研究中，我们旨在研究在卵巢癌细胞的侵袭和增殖中的作用。我们使用卵巢癌细胞进行了细胞活力、集落形成和侵袭测定，这些细胞用 siRNA 处理以敲低基因。通过抑制后用 Western blot 评估对涉及凋亡、Wnt、Hippo 和视网膜母细胞瘤信号通路的蛋白的调控作用。此外，我们分析了基因表达谱交互式分析中可用的数据，以分析与、β、、、和之间的相关性。与非恶性组织相比，在卵巢癌细胞系和样本中高表达。下调抑制细胞活力和侵袭，并促进 YAP、GSK-3-β、β-连环蛋白和视网膜母细胞瘤的磷酸化。然而，与对照相比，细胞周期蛋白 D1、cdk4 和 caspase-9 下调。总体而言，通过调节 Hippo 和 Wnt 途径促进卵巢癌发生。
