Lindström P, Palmblad J
Department of Medicine III, Karolinska Institute, Stockholm, Sweden.
J Clin Lab Immunol. 1988 Feb;25(2):77-81.
We studied adherence and chemotaxis of peritoneal neutrophils from rats fed a diet providing 0.3, 3 or 10% of the energy as linoleic acid. Whereas neutrophils from the 0.3% group showed biochemical evidence of essential fatty acid deficiency (EFAD), cells from the 10% group (the high essential fatty acid (HEFA) group) exhibited a 38% increase of linoleic acid compared to the control group (3% group). EFAD neutrophils showed a significantly lower hyperadherence in response to the ionophore A23187 relative to controls. Also, the fMLP induced response was slightly lower, whereas adherence responses to PMA were unaffected. fMLP induced chemotaxis was only 42% of controls. In HEFA neutrophils, hyperadherence to PMA was significantly diminished, and fMLP or ionophore A23187 stimulation resulted only in a slightly lower hyperadherence compared to controls. Chemotaxis in response to fMLP was significantly decreased. Spontaneous adherence and migration as well as hyperadherence and chemotaxis in response to LTB4 did not differ from controls for any of the groups. This study shows that manipulation of the dietary content of linoleic acid can alter neutrophil functions such as adherence and chemotaxis.
我们研究了以亚油酸提供0.3%、3%或10%能量的饮食喂养的大鼠腹膜中性粒细胞的黏附性和趋化性。0.3%组的中性粒细胞显示出必需脂肪酸缺乏（EFAD）的生化证据，而10%组（高必需脂肪酸（HEFA）组）的细胞与对照组（3%组）相比，亚油酸增加了38%。与对照组相比，EFAD中性粒细胞对离子载体A23187的反应显示出显著更低的高黏附性。此外，fMLP诱导的反应略低，而对PMA的黏附反应未受影响。fMLP诱导的趋化性仅为对照组的42%。在HEFA中性粒细胞中，对PMA的高黏附性显著降低，与对照组相比，fMLP或离子载体A23187刺激仅导致略低的高黏附性。对fMLP的趋化性显著降低。任何组对LTB4的自发黏附、迁移以及高黏附性和趋化性与对照组均无差异。本研究表明，改变饮食中亚油酸的含量可改变中性粒细胞的功能，如黏附性和趋化性。
