Bakala H, Cornet S, Cheignon M, Djaziri R, Schaeverbeke J
Laboratoire de Biologie Cellulaire, Université Paris, France.
J Morphol. 1988 Apr;196(1):1-14. doi: 10.1002/jmor.1051960102.
Proteoglycans were studied in developing rat fetal kidney using cytological and biochemical techniques. These compounds were detected with immunoperoxidase in the nephron basement membranes from the earliest stages of differentiation. In the glomerular basement membrane, immunostaining appeared as both diffuse and granular deposits, as long as this membrane consisted of loose material; however, as soon as a three-layered membrane had formed, staining was confined to the laminae rarae as regularly arranged granules. The same pattern of staining was observed during differentiation of the basement membrane of the proximal tubule. In Bowman's capsule, immunostaining appeared as granules, which were sparsely distributed in the developing glomerulus and then regularly lined the stacked laminae when differentiation was complete. In all basement membranes, anionic sites (disclosed by polyethyleneimine) were colocated with immunostained granular deposits. Total glycosaminoglycan content gradually increased from the beginning of metanephros development to birth. During this period, the relative proportions of glycosaminoglycans changed: heparan sulfate increased and hyaluronic acid decreased as differentiation proceeded. The possible relationship between morphological observations and biochemical changes in glycosaminoglycan content is discussed.
利用细胞学和生化技术对发育中的大鼠胚胎肾脏中的蛋白聚糖进行了研究。从分化的最早阶段开始，就在肾单位基底膜中通过免疫过氧化物酶检测到了这些化合物。在肾小球基底膜中，只要该膜由疏松物质组成，免疫染色就表现为弥漫性和颗粒状沉积物；然而，一旦形成三层膜，染色就局限于呈规则排列颗粒的透明板。在近端小管基底膜分化过程中观察到相同的染色模式。在鲍曼囊内，免疫染色表现为颗粒，这些颗粒在发育中的肾小球中稀疏分布，分化完成时则规则地排列在堆叠的板层上。在所有基底膜中，阴离子位点（由聚乙烯亚胺揭示）与免疫染色的颗粒沉积物共定位。从后肾发育开始到出生，总糖胺聚糖含量逐渐增加。在此期间，糖胺聚糖的相对比例发生了变化：随着分化的进行，硫酸乙酰肝素增加，透明质酸减少。讨论了形态学观察与糖胺聚糖含量生化变化之间的可能关系。
