Department of Studies in Microbiology, Davangere University, Shivagangothri, Davangere, Karnataka, 577007, India.
Mol Biol Rep. 2021 Mar;48(3):2389-2397. doi: 10.1007/s11033-021-06272-6. Epub 2021 Mar 18.
Lichens comprise highly diverse and complex microbial communities, the majority consisting of mycobiont, photobiont, Basidiomycetes yeast and bacteriobiont (internal bacterial communities). In this study, bacterial diversity of foliose lichen was reported. Next generation sequence (NGS) such as Illumina Sequencing (150*2) of 16S rRNA (V3 and V6 region) was used to delineate the bacterial communities associated with five foliose lichen samples. Bacterial sequences obtained from lichen samples suggested that, they harboured bacterial community with variable relative abundances. Among all bacterial communities, Alphaproteobacteria were dominant in all the tested lichen samples. The principal coordinate analysis, Venn and bar chart showed significant microbial changes between the different useful bacterial lineages across the lichens. The relative abundance of dominant and rare bacterial species found were varied, diverse, distinct and unique in each lichen. The Proteobacteria 48.19%, Actinobacteria 25.70%, Bacteroidetes 8.53%, Acidobacteria 9.36% and Chloroflexi 0.83% were predominant in all tested lichens. The present empirical study enhances the confirmed knowledge of bacterial diversity inevitably associated with lichens and is the first report on lichenized bacterial diversity and perhaps their potential possible role in lichen symbiosis in addition to phycobiont and mycobiont.
地衣包含高度多样化和复杂的微生物群落，其中大多数由菌根、光合生物、担子菌酵母和细菌共生体（内部细菌群落）组成。本研究报道了叶状地衣的细菌多样性。采用下一代测序（NGS），如 Illumina 测序（150*2）对 16S rRNA（V3 和 V6 区）进行测序，以描绘与五个叶状地衣样本相关的细菌群落。从地衣样本中获得的细菌序列表明，它们含有具有不同相对丰度的细菌群落。在所有的细菌群落中，α变形菌在所有测试的地衣样本中都占优势。主坐标分析、Venn 和条形图显示，不同有用的细菌谱系在地衣之间存在显著的微生物变化。在每个地衣中，优势和稀有细菌物种的相对丰度都有所不同，多样，独特。所有测试的地衣中，变形菌门 48.19%，放线菌门 25.70%，拟杆菌门 8.53%，酸杆菌门 9.36%和绿弯菌门 0.83%占优势。本实证研究增强了与地衣相关的细菌多样性的确认知识，这是对地衣化细菌多样性的首次报道，除了藻共生体和菌根共生体外，它们在地衣共生中的潜在作用也可能得到证实。
