School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong Province, China.
School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong Province, China.
Food Chem. 2021 Aug 30;354:129526. doi: 10.1016/j.foodchem.2021.129526. Epub 2021 Mar 10.
Determining the animal source in meat and meat products is crucial to prevent meat adulteration and fraud. Conventional methods require considerable operator skills, expensive instruments and are unable to provide fast mobile on-site detection systems to detect contamination of meat products. We developed a visual method based on recombinase polymerase amplification (RPA) and a lateral flow dipstick (LFD) to identify beef (Bos taurus), sheep (Ovis aries), pork (Sus scrofa), duck (Anas platyrhynchos) and chicken (Gallus gallus). The reaction was completed within 20 min. The results were determined by the naked eye. The detection limits of the RPA-LFD assays for duck, beef, sheep, chicken and pork were 10/µL, 10/µL, 10/µL, 10/µL and 10/µL, respectively. Furthermore, the RPA-LFD assays could differentiate species in boiled, microwaved, pressure-cooked or fried samples. These RPA-LFD assays represent a rapid, mobile detection system for determining meat product contamination.
确定肉类和肉类产品中的动物来源对于防止肉类掺假和欺诈至关重要。传统方法需要相当的操作人员技能、昂贵的仪器，并且无法提供快速的移动现场检测系统来检测肉类产品的污染。我们开发了一种基于重组酶聚合酶扩增（RPA）和横向流动纸条（LFD）的可视化方法，用于鉴定牛肉（Bos taurus）、羊肉（Ovis aries）、猪肉（Sus scrofa）、鸭肉（Anas platyrhynchos）和鸡肉（Gallus gallus）。反应在 20 分钟内完成。结果通过肉眼判断。RPA-LFD 检测法对鸭肉、牛肉、羊肉、鸡肉和猪肉的检测限分别为 10/µL、10/µL、10/µL、10/µL 和 10/µL。此外，RPA-LFD 检测法可以区分煮、微波、加压煮或炸过的样品中的物种。这些 RPA-LFD 检测法代表了一种快速、移动的检测系统，可用于确定肉类产品的污染。
