Department of Biosciences, COMSATS University Islamabad (CUI), Park Road, Islamabad, Pakistan; Food Colloids and Bioprocessing Group, School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK.
Department of Biosciences, COMSATS University Islamabad (CUI), Park Road, Islamabad, Pakistan.
Food Chem. 2021 Aug 15;353:129505. doi: 10.1016/j.foodchem.2021.129505. Epub 2021 Mar 9.
The impact of encapsulating carvacrol in chitosan-albumin based core-shell nano-carriers (NCs) on its stability and bioaccessibility was determined under simulated digestion conditions. These NCs consisted of chitosan (C) core enclosed by bovine serum albumin (BSA) shell. The mean particle size ranged from 52.4 ± 10 nm to 203 ± 6 nm and zeta-potential from + 21 ± 3.6 to -18 ± 2.7 mV. The size and charge were significantly modified after the protein-shell formation around the polysaccharide-core. Core-shell NCs were more stable, with less aggregation under simulated gastrointestinal conditions than C-NCs, presumably due to greater steric repulsion. Likewise, core-shell NCs were observed relatively more stabilized in the intestinal phase than gastric phase. The bioaccessibility of carvacrol was enhanced significantly when it was encapsulated in the core-shell NCs. These findings imply that C-BSA based core-shell NCs might be an efficient means of encapsulating, protecting and delivering hydrophobic bioactive compounds for applications in functional foods.
在模拟消化条件下，研究了将香芹酚包封在壳聚糖-白蛋白基核壳纳米载体（NCs）中对其稳定性和生物利用度的影响。这些 NCs 由壳聚糖（C）核和牛血清白蛋白（BSA）壳组成。平均粒径范围为 52.4±10nm 至 203±6nm，zeta 电位为+21±3.6mV 至-18±2.7mV。在多糖核周围形成蛋白质壳后，粒径和电荷发生了显著变化。与 C-NCs 相比，核壳 NCs 在模拟胃肠道条件下更稳定，聚集程度更低，这可能是由于空间排斥作用更大。同样，在肠道相中，核壳 NCs 比在胃相中更稳定。当香芹酚被包封在核壳 NCs 中时，其生物利用度显著提高。这些发现表明，基于 C-BSA 的核壳 NCs 可能是一种有效的包封、保护和递送疏水性生物活性化合物的方法，可用于功能性食品。
