Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China.
Food Funct. 2020 Feb 26;11(2):1525-1536. doi: 10.1039/c9fo01998g.
Resveratrol (RES)-loaded protein-polysaccharide nanoparticles were fabricated through simple electrostatic interactions with oppositely charged α-lactalbumin (ALA) and chitosan (CHI) with a mass ratio of 5 : 1 without the addition of NaCl at pH 6.5. The Z-average diameter and zeta-potential values of RES-ALA-CHI nanoparticles were 211.0 nm and 13.23 mV, respectively. Both TEM and AFM graphs confirmed that RES-ALA-CHI nanoparticles had a spherical shape, and were dispersed homogeneously at the nanoscale. The encapsulation efficiency (EE) and loading amount (LA) of RES in RES-ALA-CHI nanoparticles were 58.86% and 196.2 μg mg-1, respectively, in the presence of 400 μg mL-1 RES. XRD results confirmed that RES was in amorphous form in ALA-CHI nanoparticles. The interaction between RES and ALA-CHI nanoparticles was mainly driven by hydrophobic interaction and hydrogen bonding. Compared to RES (free), the UV light and heat stability, in vitro bioaccessibility, and antioxidant activity of RES in RES-ALA-CHI nanoparticles were pronouncedly enhanced. The information provided in this study should be of interest to the food industry to fabricate robust nanoscale delivery systems with ALA-CHI nanoparticles for RES and other hydrophobic bioactive compounds.
通过在 pH 6.5 时简单的静电相互作用，使用质量比为 5：1 的带相反电荷的α-乳白蛋白（ALA）和壳聚糖（CHI）来制备负载白藜芦醇（RES）的蛋白-多糖纳米粒子，而无需添加 NaCl。RES-ALA-CHI 纳米粒子的 Z 均粒径和 Zeta 电位值分别为 211.0nm 和 13.23mV。TEM 和 AFM 图谱均证实 RES-ALA-CHI 纳米粒子呈球形，在纳米尺度上均匀分散。在 400μg mL-1 RES 的存在下，RES-ALA-CHI 纳米粒子中 RES 的包封效率（EE）和载药量（LA）分别为 58.86%和 196.2μg mg-1。XRD 结果证实 RES 在 ALA-CHI 纳米粒子中呈无定形形式。RES 与 ALA-CHI 纳米粒子之间的相互作用主要由疏水相互作用和氢键驱动。与 RES（游离态）相比，RES 在 RES-ALA-CHI 纳米粒子中的紫外光和热稳定性、体外生物可及性和抗氧化活性显著增强。本研究提供的信息应该引起食品工业的兴趣，以制造具有 ALA-CHI 纳米粒子的稳健的纳米级递送系统，用于 RES 和其他疏水性生物活性化合物。
