Division of Epidemiology, Biostatistics, & Environmental Health, School of Public Health, University of Memphis, Memphis, TN 38152, USA.
Human Development & Health, Faculty of Medicine, University of Southampton, Southampton, SO17 1BJ, UK.
Epigenomics. 2021 Apr;13(7):485-498. doi: 10.2217/epi-2020-0384. Epub 2021 Mar 19.
Agreement in DNA methylation (DNAm) at the genome scale between blood leukocytes (BL) and bronchial epithelial cells (BEC) is unknown. We examine as to what extent DNAm in BL is comparable with that in BEC and serves as a surrogate for BEC. Overall agreement (paired -tests with false discovery rate adjusted p > 0.05) and consistency (Pearson's correlation coefficients >0.5) between two tissues, at each of the 767,412 CpGs, were evaluated. We identified 247,721 CpGs showing overall agreement and 47,371 CpGs showing consistency in DNAm. Identified CpGs are involved in certain immune pathways, indicating the potential of using blood as a biomarker for BEC at those CpGs in lower airway-related diseases. CpGs showing overall agreement and those without overall agreement are distributed differently on the genome.
血液白细胞（BL）和支气管上皮细胞（BEC）之间在全基因组水平上的 DNA 甲基化（DNAm）一致性尚不清楚。我们研究了 BL 中的 DNAm 在多大程度上与 BEC 中的 DNAm 具有可比性，并可作为 BEC 的替代物。在 767412 个 CpG 中的每一个，我们评估了两种组织之间的总体一致性（经过错误发现率调整的配对检验，p > 0.05）和一致性（Pearson 相关系数> 0.5）。我们鉴定出 247721 个 CpG 表现出总体一致性，47371 个 CpG 表现出 DNAm 的一致性。鉴定出的 CpG 参与某些免疫途径，这表明在与下呼吸道相关的疾病中，使用血液作为 BEC 中这些 CpG 的生物标志物具有潜力。表现出总体一致性的 CpG 与没有总体一致性的 CpG 在基因组上的分布不同。
