Department of Nursing, Faculty of Health Sciences, 187438Giresun University, Turkey.
Faculty of Health Sciences, 187438Giresun University, Turkey.
Biol Res Nurs. 2021 Oct;23(4):568-574. doi: 10.1177/10998004211003668. Epub 2021 Mar 19.
Diabetes mellitus is a serious chronic disease in which the oxidant-antioxidant balance is impaired, causing many complications, including hepatopathy. In this study, the effects of short-term and low-dose (NAC) administration on the biochemical, proinflammatory, and oxidative stress parameters in the liver tissue of diabetic rats were investigated. Twenty-four adult male Wistar albino rats weighing approximately 250-300 g were divided into 4 groups (n = 6): Control, Streptozotosin (STZ)-induced diabetes (DM), NAC treatment (60 mg/kg), and STZ-induced diabetes treated with NAC (DM+NAC; 60 mg/kg). NAC treatment was administered intraperitoneally as a single daily dose for 7 days. At the end of the experiment (3 weeks), blood and liver samples were collected for biochemical parameter analysis. Lipid peroxidation, antioxidant parameters, and nitric oxide (NOx) levels were determined by spectrophotometric method. Tissue inflammation parameters were evaluated by ELISA. Lipid peroxidation, proinflammatory cytokines, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) values increased significantly with diabetes. NAC treatment significantly decreased serum ALT and AST levels and proinflammatory cytokines in the diabetic group. Liver glutathione (GSH) and NOx levels increased significantly in the DM+NAC group ( 0.05). While NAC treatment reduced lipid peroxidation in the liver, it improved the inflammatory response and antioxidant status. The beneficial effect of NAC treatment may be due to its antioxidant activity and the resulting increased level of GSH. The results show that low-dose and short-term NAC treatment had a positive effect on oxidative damage and inflammation in liver tissue. NAC can be used as a potential antioxidant in diabetes to prevent hepatopathy.
糖尿病是一种严重的慢性疾病，其中氧化还原平衡受到损害，导致许多并发症，包括肝病变。在这项研究中，研究了短期和低剂量 (NAC) 给药对糖尿病大鼠肝组织生化、促炎和氧化应激参数的影响。将 24 只成年雄性 Wistar 白化大鼠（体重约 250-300g）分为 4 组（n = 6）：对照组、链脲佐菌素 (STZ) 诱导的糖尿病 (DM)、NAC 治疗组 (60mg/kg) 和 NAC 治疗的 STZ 诱导糖尿病组 (DM+NAC; 60mg/kg)。NAC 治疗通过腹腔内单次每日剂量给药 7 天。在实验结束时（3 周后），采集血液和肝脏样本进行生化参数分析。通过分光光度法测定脂质过氧化、抗氧化参数和一氧化氮 (NOx) 水平。通过 ELISA 评估组织炎症参数。糖尿病使脂质过氧化、促炎细胞因子、丙氨酸氨基转移酶 (ALT) 和天冬氨酸氨基转移酶 (AST) 值显著升高。NAC 治疗可显著降低糖尿病组血清 ALT 和 AST 水平和促炎细胞因子。DM+NAC 组肝谷胱甘肽 (GSH) 和 NOx 水平显著升高（P < 0.05）。虽然 NAC 治疗降低了肝脏的脂质过氧化，但它改善了炎症反应和抗氧化状态。NAC 治疗的有益作用可能归因于其抗氧化活性和由此产生的 GSH 水平升高。结果表明，低剂量和短期 NAC 治疗对肝组织氧化损伤和炎症有积极影响。NAC 可作为糖尿病的潜在抗氧化剂，用于预防肝病变。
